Project description:Turmeric, is both a culinary spice but also used it traditional medicines and dietary supplements for a growing list of disorder including arthritis, digestive disorders respiratory infections, allergies, liver disease, depression. The activities of turmeric are commonly attributed to curcuminoids. To understand this, In this study we compare the alters epigenetic H3K27Ac marks caused by curcupheols and cannabinoids in tissue culture cell lines.
Project description:At present, with the accelerating pace of life, increase in work pressure, lack of exercise, irregularity of diet and rest, and problems of environmental pollution, people with immunodeficiency are gradually increasing in the modern population. Some Chinese herbal medicines can improve the immunity of the human body, and have less and mild side or toxic effects, so that the study on this kind of traditional Chinese medicines and its mechanism has become an urgent problem to be solved. In recent years, studies have shown that Shcisandrol A (Sch A) can regulate immune function and inhibit the inflammation of nervous system. In this study, mRNA expression profile chip was used to screen the differentially expressed genes related to the effect of Sch A on immunodepressed mice induced by cyclophosphamide (Cy), and the differentially expressed gene-telated pathways were analyzed by gene ontology function cluster analysis, and finally qPCR was applied to verify the 5 genes that might be related to the regulation of Sch A on the mice’s immune functions, to provide a theoretical basis for screening the drug targets on which Sch A could act to regulate the mice’s immune functions.
Project description:Traditional Chinese medicines (TCM), usually composed of a mixture of components, may simultaneously target multiple genes/pathways and thus achieve superior efficacy for complex diseases such as cancer. To identify novel mechanisms of action and potential health benefits for a TCM formula Si-Wu-Tang (SWT) widely used for women’s health, we obtained the DNA microarray expression profiles for SWT, its active component ferulic acid, and estradiol in human breast cancer cell line MCF-7 and analyzed the gene expression signatures associated with each treatment using the “Connectivity Map” (cMAP). This study indicates that DNA microarray profiling analysis and cMAP data mining provide a powerful approach to discover unknown mechanisms of actions and identify potential new health benefits for TCM.