Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide ChIP-seq analysis of H2A.Z and H2A.Zac in HeLa Kyoto cells upon knockdown of JAZF1

ABSTRACT: Genome-wide ChIP-seq analysis of H2A.Z and H2A.Zac in HeLa Kyoto cells upon knockdown of JAZF1

PROVIDER: PRJNA685752 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide ChIP-seq analysis of H2A.Z and H2A.Zac in HeLa Kyoto cells upon knockdown of JAZF1

Project description:The goal was to investigate genome-wide if JAZF1 influences the deposition as well as the acetylation of the histone variant H2A.Z. ChIP-seq analysis revealed that depletion of JAZF1 leads to reduced acetylation of H2A.Z at specific sites.

2021-02-22 | GSE163318 | GEO

Gene expression analysis in HeLa Kyoto cells upon knockdown of JAZF1

Project description:Gene expression analysis in HeLa Kyoto cells upon knockdown of JAZF1

| PRJNA685204 | ENA

Gene expression analysis in HeLa Kyoto cells upon knockdown of JAZF1

Project description:The goal was the investigation of JAZF1's possible role in regulating gene expression. RNA-seq analysis revealed that depletion of JAZF1 results in transcriptome changes that affect, among others, ribosome biogenesis.

2021-01-16 | GSE163214 | GEO

Microarray upon ZMYND8 knockdown in Hela cells

Project description:Microarray upon ZMYND8 knockdown in Hela cells

| PRJNA418410 | ENA

m6A-RIP-seq of heat shock-exposed HeLa cells upon HSATIII knockdown

Project description:To investigate the effect of HSATIII lncRNA on m6A modification, we performed m6A-RIP(RNA immuno precipitation) RNA-seq from heat shock-exposed HeLa cells upon HSATIII knockdown.

2021-04-30 | GSE164200 | GEO

Microarray upon ZMYND8 knockdown in Hela cells

Project description:ZMYND8, a chromatin reader/effector protein, knockdown in Hela cells

2018-11-09 | GSE106900 | GEO

H2A.Z knockdown RNAseq

Project description:This experiment seeks to ascertain the transcriptional changes in the adult mouse hippocampus (CA1 subregion) that occur following viral knockdown of the histone variant H2A.Z. We are especially interested in understanding the role of this histone variant in memory formation and memory maintenance in the adult central nervous system. This experiment includes 3 groups, each with 3 biological replicates. Samples S108, S109, and S110 are from controls infected with an AAV expressing a scrambled shRNA control. Samples A100, A101, A102, A104, A106, and A107 were infected with an AAV expressing an shRNA against H2A.Z. Samples A100, A101, and A102 were naive animals, whereas samples A104, A106, and A107 were trained in contextual fear conditioning.

2014-09-09 | E-GEOD-58797 | biostudies-arrayexpress

Effect of modified H2A.Z protein to gene expression in DT40 cells.

Project description:H2A.Z-nucleosomes participate in both euchromatin and heterochromatin and it has proven difficult to reveal how the disparate roles and stability features imparted by H2A.Z are connected. Using an in situ assay of nucleosome stability in nuclei of DT40 cells expressing engineered forms of the variant we show that H2A.Z is released from nucleosomes of peripheral heterochromatin at unusually high salt concentrations, compared to cells expressing C-terminally truncated H2A.Z. Binding of the tail-peptide (C9) to reconstituted nucleosomes, DNA and the nuclear lamina were detected. Upon treatment of HeLa nuclei with C9, the H2A.Z-nucleosomes assumed canonical stability, the peripheral heterochromatin became dispersed and overall nuclease sensitivity increased, recapitulating tail-dependent differences in DT40. When introduced into live cells, C9 elicited chromatin reorganization and transcriptional down-regulation of ~600 genes. Thus, large-scale epigenetic modulation can be achieved by targeting or making advantage of molecular interactions involving the C-terminal tail of H2A.Z.

2023-02-23 | GSE225680 | GEO

Genome-wide maps of histone H2A.Z and its chaperone.

Project description:We use ChIP-Seq technology to profile the occupancy of histone H2A.Z and its chaperone in HeLa cells genome wide to check if they are co-colocalized. H2A.Z is ChIPed by antibody and its chaperone is tagged by Flag and ChIPed by M2 beads against Flag. ChIPed fragments are sequenced by Illumina HiSeq 2000 platform.

2014-03-01 | E-GEOD-52371 | biostudies-arrayexpress

Transcriptome landscape of HeLa response upon triamcinolone acetonide

Project description:Transcriptome landscape of HeLa response upon triamcinolone acetonide

| PRJNA475732 | ENA