Project description:We examine the chromatin landscape of murine CD4+ T cell cultured under Th0 condition (anti-CD3; anti-CD28; IL-2), treated with DMSO, isoalloLCA (20 uM) for 48 hrs, We compare cells from wild-type control mice and Nr4a1-deficient mice

2021-01-06 | GSE163350 | GEO

RNAseq and ATACseq Analysis CD4+ T cells response to isoalloLCA treatment in vitro

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-01-06 | GSE163351 | GEO

RNAseq Analysis CD4+ T cells response to isoalloLCA treatment in vitro

Project description:We examine the transcription response of murine CD4+ T cell cultured under Th0 condition (anti-CD3; anti-CD28; IL-2), treated with DMSO, isoalloLCA (20 uM) for 48 hrs, or in the presence of TGFbeta (0.25 ng/mL). We compare the differential genes regulated by isoalloLCA and TGFbeta.

2021-01-06 | GSE163349 | GEO

CD4+ T cell response to IL12 in vitro

Project description:Here we analyzed the transcriptional response to IL12 in activated CD4+ T cells in vitro. Splenic CD4+ T cells were isolated by negative selection from 6wk old male C57BL/6 mice. These were activated with CD3/28 beads for 48hrs and thereafter cultured with IL12 (100U/ml) for 2hrs or 8hrs in vitro. Cells were then transferred directly into TriZol. RNA was prepared in Trizol for gene expression profiling by Affymetrix Mouse Gene 1.0 ST Arrays.

2016-07-20 | E-GEOD-84567 | biostudies-arrayexpress

RNAseq of naive CD4 T cells deficient for Elp3 activated for 18h against WT control

Project description:CD4 T cell deficient for the Elp3 gene have a delayed cell cycle entry. We explored by RNAseq by which molecular pathway the absence of Elp3 delayed the cell cycle entry. Naive T cell from CD4-cre Elp3 fl/fl mice and WT littermate were purified and activated in vitro with CD3 and CD28 antibodies for 18h.

2020-07-31 | E-MTAB-9057 | biostudies-arrayexpress

CD4+ T cell response to IFNa in vitro

Project description:Here we analyzed the transcriptional response to IFNa in activated CD4+ T cells in vitro. We found robust induction of ISGs as early as 2hrs. Splenic CD4+ T cells were isolated by negative selection from 6wk old male C57BL/6 mice. These were activated with CD3/28 beads for 48hrs and thereafter cultured with IFNa (100U/ml) for 2hrs or 8hrs in vitro. Cells were then transferred directly into TriZol. RNA was prepared in Trizol for gene expression profiling by Affymetrix Mouse Gene 1.0 ST Arrays.

2016-07-20 | E-GEOD-84566 | biostudies-arrayexpress

CD4+ T cell response to IL12 in vitro

Project description:Here we analyzed the transcriptional response to IL12 in activated CD4+ T cells in vitro.

2016-07-20 | GSE84567 | GEO