Project description:Two independent small RNA (sRNA) libraries from male and female asparagus plants were sequenced, generating 4.13 and 5.88 million final cleaned reads, respectively. A total of 154 conserved miRNA belonging to 26 families, and 40 novel miRNA candidates that seemed to be specific to asparagus were identified, among them, 63 miRNAs exhibited significant differential expression between male and female plants, and 36 target mRNAs representing 44 conserved and fournovel miRNA in asparagus by high-throughput degradome sequencing analysis. Examination of small RNA in male and female asparagus using high-throughput sequencing

2015-09-02 | E-GEOD-72594 | biostudies-arrayexpress

Asparagus officinalis

Project description:Asparagus officinalis small RNA sequencing

| PRJNA357937 | ENA

Identification of miRNAs and its targets using high-throughput sequencing and degradome analysis in male and female Asparagus officinalis.

2015-09-02 | GSE72594 | GEO

Small RNA (sRNA) sequencing data from pre-meiotic and meiotic anthers of Asparagus

Project description:Purpose: To study pre-meiotic (21-nt) and meiotic (24-nt) phasiRNA pathways in non-grass monocots Methods: Anthers were dissected using a 2 mm stage micrometer in a stereo microscope, and immediately frozen in liquid nitrogen until total RNA isolation was performed. Small RNA, mRNA libraries were generated using short-read (Illumina) and Single Molecule Real Time SMRT (PacBio) sequencing approaches. Stages were assigned based on the morphology of archesporial (AR) and tapetal cells of A. officinalis (Asparagus) anthers.

2018-07-07 | GSE112800 | GEO

The asparagus genome sheds light on the origin and evolution of a young Y chromosome

Project description:Sex chromosomes evolved from autosomes many times across the eukaryote phylogeny. Several models have been proposed to explain this transition, some involving male and female sterility mutations linked in a region of suppressed recombination between X and Y (or Z/W, U/V) chromosomes. Comparative and experimental analysis of a reference genome assembly for a double haploid YY male garden asparagus (Asparagus officinalis L.) individual implicates separate but linked genes as responsible for sex determination. Dioecy has evolved recently within Asparagus and sex chromosomes are cytogenetically identical with the Y, harboring a megabase segment that is missing from the X. We show that deletion of this entire region results in a male-to-female conversion, whereas loss of a single suppressor of female development drives male-to-hermaphrodite conversion. A single copy anther-specific gene with a male sterile Arabidopsis knockout phenotype is also in the Y-specific region, supporting a two-gene model for sex chromosome evolution. Additionally, we test for the presence of Y-specific small RNA loci in several XX, XY, and YY genotypes that may be acting as sex determination loci.

2020-05-02 | GSE149730 | GEO

Asparagus officinalis

Project description:Asparagus officinalis flower organ RNA sequencing

| PRJNA435625 | ENA

Transcriptome analysis during the growth and development of asparagus stems

Project description:Asparagus is a nutritionally dense stem vegetable whose growth and development are correlated with its quality and yield. For height of 25 cm asparagus , 17-20 cm is still viable, in which the cells can continue to elongate. When 40 cm, the growth in the 17-20 cm range basically stops. Therefore, we picked periods with significant growth differences for transcriptome comparison to find the dynamic changes and underlying mechanisms during the elongation and growth process of asparagus stems. The results showed that the differentially expressed genes were enriched in many aspects, including the metabolism of plant hormones and carbohydrates.

2024-02-29 | GSE252560 | GEO

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