Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Nucleocytoplasmic Transport of RNA-Binding Proteins Regulates Neural Stem Cell Fates

ABSTRACT: Nucleocytoplasmic Transport of RNA-Binding Proteins Regulates Neural Stem Cell Fates

PROVIDER: PRJNA687227 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Nucleocytoplasmic Transport of RNA-Binding Proteins Regulates Neural Stem Cell Fates

Project description:The formation of the cerebral cortex requires balanced expansion and differentiation of neural progenitor cells, the fate choice of which requires regulation at many steps of gene expression. As progenitor cells often exhibit transcriptomic stochasticity, the ultimate output of cell fate-determining genes must be carefully controlled at the post-transcriptional level, but how this is orchestrated is poorly understood. Here, we report that de novo variants in an RNA-binding protein CELF2 cause human cortical malformations and perturb neural progenitor cell fate decisions in mice by disrupting the nucleocytoplasmic transport of CELF2. In self-renewing neural progenitors, CELF2 is localized in the cytoplasm where it binds and coordinates mRNAs that encode cell fate regulators and neurodevelopmental disorder-related factors. The translocation of CELF2 into the nucleus releases mRNAs for translation and thereby triggers neural progenitor differentiation. Our results reveal a mechanism by which transport of CELF2 between discrete subcellular compartments orchestrates an RNA regulon to instruct cell fates in cerebral cortical development.

2021-01-11 | GSE163703 | GEO

ATAC-seq on ESCs undergoing directed differentiation towards defined neural fates

Project description:We have used ATAC-seq to track cell state changes that occur during the differentiation of mouse embryonic stem cells to defined neural progenitor fates. We have performed ATAC-seq every 24 hours in cells en route to 3 distinct neural progenitors fates, anterior, hindbrain and spinal cord. This has allowed us to define how cells transition to a neural state, based on their enhancer usage. We identified regions distinct to different anterior-posterior neural progenitors, and validated their relevance by performing in vivo ATAC-seq on neural progenitors isolated from different axial levels of mouse embryos.

2018-08-27 | E-MTAB-6337 | biostudies-arrayexpress

ATAC-seq on ESCs undergoing directed differentiation towards defined neural fates

Project description:ATAC-seq on ESCs undergoing directed differentiation towards defined neural fates

| PRJEB27586 | ENA

RNA-binding proteins govern neural progenitor hierarchy during development and tumorigenesis

Project description:RNA-binding proteins govern neural progenitor hierarchy during development and tumorigenesis

| PRJNA471745 | ENA

Multiomics Analyses of Vesicular Transport Pathway-Specific Transcripts and Proteins in Ovine Amnion: Responses to Altered Intramembranous Transport

Project description:Amniotic fluid (AF) volume (AFV) is determined primarily by the rate of intramembranous (IM) transport of AF across the amnion. Intramembranous transport is characterized as vesicular endocytotic and transcytotic processes regulated by fetal urine-derived stimulators and AF inhibitors. Our objectives were to utilize a large-scale multiomics approach to decipher the vesicular transport pathways in the amnion and to identify potential transport regulators in AF and fetal urine. We utilized fetal sheep to experimentally induce alterations in IM transport rate and analyze the expression profiles of transcripts and proteins in amnion, AF and fetal urine. Four experimental groups were studied: control (C), urine drainage with reduced IM transport rate and AFV (UD), urine drainage and fluid replacement with decreased IM transport rate but increased AFV (UDR), and intra-amniotic fluid infusion with augmented IM transport rate and AFV (IA). Amnion and fluid samples were subjected to transcriptomics (RNA-Seq) and proteomics studies followed by bioinformatics analyses using Ingenuity Pathway Analysis software. The analyses revealed 9 functional transport pathways and a panel of differentially expressed transcripts and proteins that are known mediators of vesicular transport and energy metabolism. During UD, expressions of endocytosis and trafficking mediators were reduced as compared to controls. Under UDR, expressions of energy metabolism activators increased while trafficking mediators decreased. During IA, expressions of vesicular uptake and transcellular trafficking regulators were enhanced. Co-expression of the motor protein, cytoplasmic dynein light chain-1, in both AF and fetal urine suggest that this molecule may function as a urine-derived stimulator of IM transport.

2019-03-30 | GSE129077 | GEO

Circulatory proteins permeate the brain via selective transport impaired with age"

Project description:Circulatory proteins permeate the brain via selective transport impaired with age"

| PRJNA553575 | ENA

Circulatory proteins permeate the brain via selective transport impaired with age

Project description:Circulatory proteins permeate the brain via selective transport impaired with age

| PRJNA597213 | ENA

Multiomics Analyses of Vesicular Transport Pathway-Specific Transcripts and Proteins in Ovine Amnion: Responses to Altered Intramembranous Transport

Project description:Multiomics Analyses of Vesicular Transport Pathway-Specific Transcripts and Proteins in Ovine Amnion: Responses to Altered Intramembranous Transport

| PRJNA529913 | ENA

Functional dissection of RanBP2 and Nup88/Nup214 subcomplex in nucleocytoplasmic transport

Project description:Functional dissection of RanBP2 and Nup88/Nup214 subcomplex in nucleocytoplasmic transport

| PRJNA550143 | ENA

ELAV/Hu RNA binding proteins determine multiple programs of neural alternative splicing

Project description:ELAV/Hu RNA binding proteins determine multiple programs of neural alternative splicing

| PRJNA688933 | ENA