Ontology highlight
ABSTRACT:
PROVIDER: PRJNA687883 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR13307828_1.fastq.gz | Fastqsanger.gz | |||
| SRR13307828_2.fastq.gz | Fastqsanger.gz | |||
| SRR13307829_1.fastq.gz | Fastqsanger.gz | |||
| SRR13307829_2.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 4 of 4 |
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Project description:Mitochondrial fusion promotor, M1, was used to study the impact of mitochondria dynamics in the female reproductive system. The M1 treatment in mice can lead to acute and reversible disruption of estrous cycles in vagina smears. The ovarian samples were collected after the control and M1 injections on the evening of proestrus. The comparative gene expression profiling analysis of RNA-seq data was performed.
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Project description:<p>The ovaries and uterus are crucial reproductive organs in mammals, and their coordinated development ensures the normal development of sexual maturity and reproductive capacity. This study aimed to comprehensively capture the different physiological stages of the goat's sexual maturation by selecting four specific time points. We collected samples of ovarian and uterine tissues from five female Jining Gray goats at each time point: after birth (D1), 2-month-old (M2), 4-month-old (M4) and 6-month-old (M6). By combining transcriptomic sequencing of 40 samples (including rRNA-depleted RNA-seq libraries with 3607.8 million reads and miRNA-seq libraries with 444.0 million reads) and metabolomics analysis, we investigated the transcriptomic mechanisms involved in reproductive regulation in the ovary and uterus during sexual maturation, as well as the changes in metabolites and their functional potential. Additionally, we analyzed blood hormone indices and uterine tissue sections to examine temporal changes. These datasets will provide a valuable reference for the reproductive regulation of the ovary and uterus, as well as the regulation of metabolites during sexual maturation in goats.</p><p><br></p><p><strong>Uterine data</strong> is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS9795' rel='noopener noreferrer' target='_blank'><strong>MTBLS9795</strong></a>.</p><p><strong>Ovarian data</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS9794' rel='noopener noreferrer' target='_blank'><strong>MTBLS9794</strong></a>.</p>
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