Project description:Transcription factor binding locations by ChIP followed by high throughput sequencing. To build and validate an automated Chromatin Immunoprecipitation and high throughput Illumina sequencing pipeline
Project description:We report the application of sequencing technology for high-throughput profiling of RUNX1 transcription factor occupancy in mouse EML cells. RUNX1 antibody was use for chromatin immunoprecipitation followed by high-throughput sequencing to reveal RUNX1 genome occupancy in hematopoietic stem/progenitor cells. Examination of RUNX1 transcription factor occupancy in EML cells.
Project description:Dense mapping of individual DNase I cleavages from intact nuclei isolated from wild type, sba1M-NM-^T and gcn5M-NM-^T yeast using high-throughput sequencing. Open chromatin status of wild type, sba1-/- and gcn5-/- yeast strains were generated by Dnase-seq.
Project description:Chromatin immunoprecipitation with antibodies specific for histone modifications H3K4me3, H3K9ac and H3K27me3 and subsequent high-throughput sequencing were performed on fixed chromatin from two septic disease patients. ChIP-seq
Project description:Chromatin immunoprecipitation against CTCF followed by Illumina High-throughput sequencing. Examination of CTCF binding in embryonic stem cells and embryonic fibroblasts
