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Single cell RNA analysis of trunk neural crest cells in zebrafish

ABSTRACT: Single cell RNA analysis of trunk neural crest cells in zebrafish

PROVIDER: PRJNA688157 | ENA |

REPOSITORIES: ENA

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Trunk neural crest gene MOXD1 affects embryonic development

Project description:Ectoderm-derived neural crest is a transient structure arising during early embryogenesis in vertebrates. Neural crest consists of four derivatives based on their anterior- to posterior location along the body axis; cranial, vagal, trunk and sacral, respectively. We recently showed that trunk neural crest-specific gene MOXD1 functions as a tumor suppressor in trunk neural crest-derived childhood cancer form neuroblastoma and is essential for proper development of healthy adrenal glands. However, the role of MOXD1 during early embryogenesis is not known. Here, we conditionally knocked out MOXD1 in trunk neural crest cells before they become lineage-committed, using a CRISPR/Cas9 approach in chick embryos. Assessment of embryo growth showed that knockout of MOXD1 delayed development with knockout embryos being smaller. RNA sequencing of trunk-derived neural crest cells from control and knockout embryos showed enrichment of genes connected to gland development, copper ion metabolism and neuroblastoma progression. In conclusion, MOXD1 is important during early and prolonged embryonic development with effects on gland formation, possibly mediated via its role in copper metabolism.

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Cancer associated HIF-2a governs trunk neural crest stemness

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Molecular events controlling cessation of trunk neural crest migration and onset of differentiation

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Identification of cranial-specific neural crest genes by comparative transcriptomics

Project description:We employ RNA-seq of FACS sorted cell populations to identify genes that are enriched in cranial neural crest in relationship to the trunk. Transcriptional profiling of delaminating cranial and trunk neural crest subpopulations.

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Ancient vertebrate dermal armor evolved from trunk neural crest

Project description:Bone is an evolutionary novelty of vertebrates, likely to have first emerged as part of ancestral dermal armor that consisted of osteogenic and odontogenic components. Whether these early vertebrate structures arose from mesoderm or neural crest cells has been a matter of considerable debate. To examine the developmental origin of the bony part of the dermal armor, we have performed in vivo lineage tracing in the sterlet sturgeon, a representative of non-teleost ray-finned fish that has retained an extensive postcranial dermal skeleton. The results definitively show that sterlet trunk neural crest cells give rise to osteoblasts of the scutes. Transcriptional profiling further reveals neural crest gene signature in sterlet scutes as well as bichir scales. Finally, histological and microCT analysis of ray-finned fish dermal armor show that their scales and scutes are formed by bone, dentin and hypermineralized covering tissues, in various combinations, that resemble those of the first armored vertebrates. Taken together, our results support a primitive skeletogenic role for the neural crest along the entire body axis, that was later progressively restricted to the cranial region during vertebrate evolution. Thus, the neural crest was a crucial evolutionary innovation driving the origin and diversification of dermal armor along the entire body axis.

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Axial progenitors generate trunk neural crest cells in vitro

Project description:Axial progenitors generate trunk neural crest cells in vitro

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Mutations in cdon and boc affect trunk neural crest cell migration and slow-twitch muscle development in zebrafish

Project description:Bulk RNA-seq of isolated slow-twtich muscle and neural crest cells from 24 hours post fertilization zebrafish.

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Axial progenitors generate trunk neural crest cells in vitro

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Single cell RNA analysis of trunk neural crest cells in zebrafish

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