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The DME demethylase regulates sporophyte gene expression, cell proliferation, differentiation, and meristem resurrection

ABSTRACT: The DME demethylase regulates sporophyte gene expression, cell proliferation, differentiation, and meristem resurrection

PROVIDER: PRJNA689568 | ENA |

REPOSITORIES: ENA

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The DME demethylase regulates sporophyte gene expression, cell proliferation, differentiation, and meristem resurrection

Project description:The flowering plant life cycle consists of alternating haploid (gametophyte) and diploid (sporophyte) generations, where the sporophytic generation begins with fertilization of haploid gametes. In Arabidopsis, genome-wide DNA demethylation is required for normal development, catalyzed by the DEMETER (DME) DNA demethylase in the gamete companion cells of male and female gametophytes. In the sporophyte, postembryonic growth and development are largely dependent on the activity of numerous stem cell niches, or meristems. Analyzing Arabidopsis plants homozygous for a loss-of-function dme-2 allele, we show that DME influences many aspects of sporophytic growth and development. dme-2 mutants exhibited delayed seed germination, variable root hair growth, aberrant cellular proliferation and differentiation followed by enhanced de novo shoot formation, dysregulation of root quiescence and stomatal precursor cells, and inflorescence meristem (IM) resurrection. We also show that sporophytic DME activity exerts a profound effect on the transcriptome of developing Arabidopsis plants, including discrete groups of regulatory genes that are misregulated in dme-2 mutant tissues, allowing us to potentially link phenotypes to changes in specific gene expression pathways. These results show that DME plays a key role in sporophytic development and suggest that DME-mediated active DNA demethylation may be involved in the maintenance of stem cell activities during the sporophytic life cycle in Arabidopsis.

2021-01-05 | GSE164217 | GEO

The DME demethylase regulates sporophyte gene expression, cell proliferation, differentiation, and meristem resurrection.

Project description: Not available

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Transcriptional analysis of Ceratopteris richardii young sporophyte reveals conservation of stem cell factors in the root apical meristem

Project description:To generate a de novo transcriptome assembly from three different parts of the Ceratopteris richardii young sporophyte (Hn-n cultivar). We then performed a differential gene expression analysis between the different plant segments, while trying to understand the expressed genes for each Ceratopteris part.

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Genome-wide transcriptomic analysis of the sporophyte of the moss Physcomitrella patens

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A Physcomitrella protein acts in spermatogenesis and sporophyte abortion

Project description:Functional targeting of PpPINC gene in Physcomitrella, researching it's role during gametangia and sporophyte development.

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Genome-wide transcriptomic analysis of the sporophyte of the moss Physcomitrella patens

Project description:Bryophytes are the most basal of the extant land plants. A major feature of these plants is the biphasic alteration of generations between a dominant haploid gametophyte and a minor diploid sporophyte phase. To analyse the differences in the transcriptome of the early gametophyte (protonema) and early and mid-sporophyte phases of the moss Physcomitrella patens, microarray gene expression profiles were performed using dissected sporophyte tissue. Through further analysis the early and mid-sporophyte phases were compared. RNA isolated from the Gametophytic protonemal tissue was hybridised to six microarrays. Each microarray was hybridised with RNA from a separate biological replicate. Three of these microarrays were co-hybridised with RNA isolated from early sporophytes. With the third gametophyte biological replicate and early sporophyte replicate a dye swap was carried out. The remaining three microarrays hybridised with RNA from the gametophytes were co-hybridised with RNA from mid-sporophytic tissue. A dye swap was carried out on the sixth gametophyte replicate and third mid-sporophyte replicate.To meet the quality requirements for the microarray experiment, at least 400 sporophytes were used per extraction. Three or four RNA extracts were then pooled for further precipitation to maximise purity and concentration. Up to 1600 sporophytes were harvested to prepare sufficient RNA for each microarray replicate. In bioinformatic analysis the channels were split into individual channels and the early and mid-sporophyte were compared.

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Altered expression of SELF-PRUNING disrupts meristem homeostasis and facilitates delivery of signals to the meristem

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Transcription profiling by high throughput sequencing of Funaria hygrometrica sporophyte and gametophyte tissue

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Microdissection: Transdifferentiation of Root Apical Meristem to Shoot Apical Meristem

Project description:gnp07_regeneome_transdifferenciation - microdissection - Study of the moleculars mecanism during transdifferenciation of Root ApicalMeristem to Shoot Apical Meristem - middle of growth permits to induce transdifferenciation of root apical meristem to shoot apical meristem

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Microdissection: Transdifferentiation of Root Apical Meristem to Shoot Apical Meristem

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