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Genome-wide analysis of gene expression in MKN7_EB by EHF knockdown (RNA-Seq).

ABSTRACT: Genome-wide analysis of gene expression in MKN7_EB by EHF knockdown (RNA-Seq).

PROVIDER: PRJNA689585 | ENA |

REPOSITORIES: ENA

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MKN7_EB by EHF knockdown

Project description:MKN7_EB by EHF knockdown

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Genome-wide analysis of gene expression in MKN7_EB by EHF knockdown (RNA-Seq).

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Project description:The cornea, composed of epithelium, stroma and endothelium, protects the anterior compartment of the eye from damage and allows transmission of light into the eye. While well described morphologically, no studies have investigated the global gene expression changes in the cornea throughout the mouseM-bM-^@M-^Ys life. We characterized the global gene expression profile of mouse cornea from early development through aging, and compared to gene expression in other epithelial tissue, to identify cornea enriched genes, pathways, and transcriptional regulators. We identified Ehf, an ets family transcription factor, as being highly selectively expressed in the corneal epithelium compared to the stroma, and highly expressed in cornea compared to other epithelial tissues. siRNA experiments and Ehf ChIP-Seq on mouse corneal epithelium confirm the role of this factor in promoting epithelial identity and cell differentiation, and suggest it carries out these functions through interactions with other cornea epithelial differentiation factors including Klf4. Whole eye globes were dissected from wild type CB6 mice. Corneal epithelium was isolated by digestion in 50% EMEM/dispase II with 50 mM sorbitol for two hours at 37M-BM-0C. ChIP was performed with an Ehf antibody, and was sequenced with an input control.

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