Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

IL-17-producing γδT cells ameliorate intestinal acute graft-versus-host disease by recruitment of Gr-1+CD11b+ myeloid-derived suppressor cells

ABSTRACT: IL-17-producing γδT cells ameliorate intestinal acute graft-versus-host disease by recruitment of Gr-1+CD11b+ myeloid-derived suppressor cells

PROVIDER: PRJNA689736 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Differentially expressed genes of CD11b+Gr-1+ myeloid cells

Project description:Differentially expressed genes of CD11b+Gr-1+ immature myeloid cells (IMCs) in the bone marrow and colonic tumor setting of histidine decarboxylase (HDC)-KO mice were examined by microarray (Affymetrix Mouse 430.2 array). Myeloid differentiation-related candidate genes were sought to be isolated and functionally studied. Total RNA of HDC-expressing CD11b+Gr-1+ IMCs of bone marrow were extracted from HDC-EGFP and HDC-EGFP/HDC-KO mice (3 mice in each group). CD11b+Gr-1+ myeloid-derived suppressor cells (MDSCs) of colon tumor were sorted from 10-12 colon tumors of WT and HDC-KO mice (5 mice in each group), and pooled to extract total RNA for microarray studies. Two technical replicates for each of the four groups. Four sets of comparisons were performed to screen for upregulated or downregulated genes in the HDC-KO CD11b+Gr-1+ IMCs or MDSCs (experiment group) compared to the WT group: (1) HDC-expressing CD11b+Gr-1+ IMCs of bone marrow of HDC KO mice compared to bone marrow IMCs of WT mice; (2) CD11b+Gr-1+ MDSCs in tumors of HDC-KO mice compared to WT mice; (3) CD11b+Gr-1+ MDSCs of WT colon tumors compared to IMCs in the WT bone marrow; and (4) CD11b+Gr-1+ MDSCs of colon tumors of HDC-KO mice compared to IMCs in the bone marrow of HDC-KO mice.

2010-12-15 | E-GEOD-23502 | biostudies-arrayexpress

Microarray analysis of splenic CD11b+Gr-1+ cells

Project description:We extracted RNA of sorted splenic CD11b+Gr-1+ cells and performed microarray analyses. Total RNA was isolated using the manufacturer’s recommended protocol for RNeasy (Qiagen, Valencia, CA). Isolated RNA was quantified, and quality was assessed using an Agilent A 2100 BioAnalyzer with the RNA NanoChip (Agilent, Andover, MA). We used 2μg of total RNA to make single-stranded antisense cDNA with the NuGEN Technologies (San Carlo, CA) Ovation Biotin System in accordance with the manufacturer’s directions. Labeled targets were hybridized to Affymetrix (Santa Clara, CA) MOE430 2.0 GeneChip microarrays for 16h at 45°C. The arrays were washed and scanned according to Affymetrix standard protocols. We used microarrays to detail the global gene expression of CD11b+Gr-1+ cells from the control mice and the clarithromycin-treated mice, and identified distinct classes of up-regulated genes during this process.

2016-05-09 | GSE81241 | GEO

Differentially expressed genes of CD11b+Gr-1+ myeloid cells

2010-12-15 | GSE23502 | GEO

IL-17-producing γδT cells ameliorate intestinal acute graft-versus-host disease by recruitment of Gr-1+CD11b+ myeloid-derived suppressor cells

Project description:We generated γδT17 cells from splenocytes and compared gene expression profiles of initial and induced γδT17 cells using RNA-seq to explore their biological functions.

2021-01-06 | GSE164262 | GEO

Transcriptomic profiles of splenic Gr-1+ cells from IDO-KO hosts and WT hosts after bone marrow transplantation

Project description:Tryptophan-catabolizing enzyme indoleamine 2,3-dioxygenase 1 (IDO1) has also immunological function to suppress T cell activation in inflammatory circumstances, including graft-versus-host disease (GVHD), a fatal complication after allogeneic bone marrow transplantation (allo-BMT). Although the mononuclear cell expression of IDO1 has been associated with improved outcomes in GVHD, the underlying mechanisms remain unclear. Herein, we used IDO-deficient (Ido1-/-) BMT to understand why myeloid IDO limits the severity of GVHD. Hosts with Ido1-/- BM exhibited increased lethality, with enhanced proinflammatory and reduced regulatory T cell responses compared with wild type (WT) alloBMT controls. Despite the comparable expression of the myeloid-derived suppressor cell (MDSC) mediators, arginase-1, inducible nitric oxide synthase, and IL-10, Ido1-/- Gr1+CD11b+ cells from allo-BMT or in vitro BM culture showed compromised immunesuppressive functions and were skewed toward the Ly6ClowLy6Ghi subset, compared with the WT counterparts. Importantly, Ido1-/-Gr-1+CD11b+ cells exhibited elevated levels of reactive oxygen species (ROS) and neutrophil numbers. These characteristics were rescued by human IDO1 with intact heme-binding and catalytic activities, and were recapitulated by the treatment of WT cells with the IDO1 inhibitor L1-methyl tryptophan. ROS scavenging by Nacetylcysteine reverted the Ido1-/-Gr-1+CD11b+ composition and function to an MDSC state, as well as improved the survival of GVHD hosts with Ido1-/- BM. In summary, myeloid-derived IDO1 enhances GVHD survival by regulating ROS levels and limiting the ability of Gr1+CD11b+ MDSCs to differentiate into proinflammatory neutrophils. Our findings provide a novel mechanistic insight into the immune-regulatory roles of the metabolic enzyme IDO1.

2021-12-20 | GSE163730 | GEO

γδT cell Interactions within the Tissue Ecosystem Network [bulk RNAseq]

Project description:Numerous lymphocytes seed the body surfaces of metazoans promoting tissue protection and integrity. These tissue-resident populations differ substantially from circulating lymphocytes, as they adopt a unique phenotype and do not recirculate. They span the innate-adaptive continuum, ranging from innate lymphoid cells (ILCs) to unconventional T cells (NKT, MAIT, γδT cells and IELs) and tissue-resident memory (TRM) T cells, and while differ in the particulars of their biology, all contribute to barrier immunity, tissue homeostasis, and immune regulation. Although they share the same microenvironment, little is known about how these tissue-resident lymphocyte populations interact with each other and with the non-lymphoid cellular environment. As such, there is an emerging view of tissues as varied ‘‘ ecosystems ’’, whereby protection against inflammatory challenges is orchestrated through the interplay between tissue-resident and infiltrating-lymphocyte subtypes. In mice, barrier tissues are populated during early life by innate-like γδT lymphocytes. These cells provide tissue protection, including limiting conventional T cell-driven inflammation. Therefore, elucidating the contributions of tissue-resident T cells, particularly γδT cells, to the basic pathophysiology of tissue function and protection against infection, inflammation, and cancer is a priority. To this aim, we have induced allergic contact dermatitis in the back skin of different strains and we analyzed the behavior of γδT cells

2022-02-01 | GSE164022 | GEO

Re-generation of cytotoxic γδT cells with distinctive signatures from human γδT-derived iPSCs

Project description:The goal of this study was to compare gene expressions on the single cell level in (i) freshly isolated PBMCs (no stimulation and no sorting), (ii) PBγδT cells; PBMCs were stimulated with HMBPP in vitro and CD3(+) γδTCR(+) cells were sorted and (iii) iγδT cells; differentiated cells from γδT-iPSC clone were stimulated with HMBPP and CD3(+) γδTCR(+) cells were sorted or not sorted.

2023-02-24 | GSE194072 | GEO

Targeted genomic sequencing of the TCRγ locus in ILC2s and γδT cells

Project description:An in-depth examination of the TCRγ locus in ILC2s and γδT cells was performed to assess the functionality of rearranged TCRγ locus.

2020-11-13 | GSE152726 | GEO

Comparison of the Gr-1+ and Gr-1- monocyte gene expression in cell-type specific Fcgr2 deficiency on the Yaa backgound

Project description:We generated 5 mouse lines:(1) Fcgr2 -/-.Yaa,(2)CD19Cre;Fcgr2 fl/fl.Yaa, (3)CEBPaCre;Fcgr2 fl/fl.Yaa, (4)CD11cCre;Fcgr2 fl/fl.Yaa, and (5)Fcgr2 fl/fl.Yaa. Peripheral blood mononuclear cells from each mouse at 8 months old were collected and sorted by CD11b and Gr-1 cell surface markers. Sorted cells were subjected to RNA extraction and RNA-seq analysis

2022-07-06 | GSE116757 | GEO