Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

SELEX and RIP-Seq identification of nucleic acid sequences bound by CIRBP

ABSTRACT: SELEX and RIP-Seq identification of nucleic acid sequences bound by CIRBP

PROVIDER: PRJNA691054 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

SELEX and RIP-Seq identification of nucleic acid sequences bound by CIRBP

Project description:SELEX (systematic evolution of ligands by exponential enrichment) and RNA Immunoprecipitation (RIP)-seq revealed that CIRBP directly binds the 3’UTR of Atp5g3.

2021-01-11 | GSE164523 | GEO

SELEX and ChIP/CLIP-Seq identification of nucleic acid sequences bound by the DDX43 KH domain (89 aa)

Project description:SELEX (systematic evolution of ligands by exponential enrichment), chromatin immunoprecipitation (ChIP)-seq, and crosslinking immunoprecipitation (CLIP)-seq showed that the DDX43 KH domain binds C/T rich DNA and U rich RNA.

2020-02-03 | GSE144647 | GEO

SELEX and ChIP/CLIP-Seq identification of nucleic acid sequences bound by the DDX43 KH domain (89 aa)

Project description:SELEX and ChIP/CLIP-Seq identification of nucleic acid sequences bound by the DDX43 KH domain (89 aa)

| PRJNA604410 | ENA

RIP-seq analysis of PTBP1-bound RNAs

Project description:To reveal PTBP1-bound RNAs, we conducted RNA immunoprecipitation sequencing (RIP-seq) with total RNA samples from MKN-28 cells.

2021-04-14 | GSE157941 | GEO

Identify Flag-bound transcripts via RIP-seq

Project description:To identify the directly bound transcripts of Flag antibody (the backgroud control for our METTL16 RIP-seq), RNA immunoprecipitation sequencing (RIP-seq) was conducted HEK293T. Briefly, HEK293T cells were infected with pmiRNA1-empty vector. Only the GFP-positive cells were used for study and expanded in DMEM medium.

2021-10-28 | GSE182620 | GEO

Identify Flag-bound transcripts via RIP-seq

Project description:Identify Flag-bound transcripts via RIP-seq

| PRJNA757015 | ENA

RIP-seq analysis of PTBP1-bound RNAs

Project description:RIP-seq analysis of PTBP1-bound RNAs

| PRJNA663450 | ENA

FLAG RIP-Seq identifies RNA species bound by cytoplasmic TAF7 in HeLa TAF7 (WT) cells

Project description:We perform FLAG RIP-Seq to generate a transcriptome-view of RNA targets bound by TAF7

2021-10-22 | GSE161667 | GEO

Selection of RNA aptamers against human islets by SELEX and toggle SELEX

Project description:Type 1 Diabetes is still an incurable disease characterized by autoimmune destruction of insulin-producing beta cells within the islet of Langerhans in the pancreas. Currently, there are no methods to monitor beta-cell mass in humans or deliver therapeutics specifically to beta cells. Here we performed Cluster Systematic Evolution of Ligands by Exponential Enrichment (SELEX) experiments and toggle SELEX experiments to identify RNA aptamers specific for human islets. In the cluster SELEX, we started from a random library of RNA nucleotides composed of a 40 nucleotide long variable region flanked by two constant regions. We performed eight selection cycles using hand-picked islets and islet-depleted acinar tissue from 4 cadaveric human donors as positive and negative selectors. In the toggle SELEX, we conducted eight cycles of selection using islets and acinar tissue from mice, followed by two cycles of selection using human tissues. The polyclonal libraries from the two selection strategies showed a convergent evolution of ligands and increased specificity for human islets.

2022-02-23 | GSE197262 | GEO

anti-TAF7 RIP-Seq identifies RNA species bound by cytoplasmic TAF7 in HeLa TAF7 (WT) cells

Project description:We perform TAF7 RIP-Seq to generate a transcriptome-view of RNA targets bound by TAF7

2021-10-22 | GSE161670 | GEO