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Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes [bulk RNA-seq]

ABSTRACT: Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes [bulk RNA-seq]

PROVIDER: PRJNA691958 | ENA |

REPOSITORIES: ENA

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Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes [bulk RNA-seq]

Project description:To identify molecular characteristics of WT AM without Wnt3a, and WT, b-catenin-deficient or HIF-1a-deficient AM with Wnt3a stimulation in vitro, we isolated RNA from AMs with various treatments and examined by bulk RNA-seq. We found a large number of gene profiles were altered following Wnt3a treatment in WT AMs, while b-catenin and HIF-1a deficiency largely abolished the effects of Wnt3a treatment on AM transcription, suggesting that b-catenin and HIF-1a mediates most downstream effects of Wnt3a in AMs. Indeed,HIF-1a-deficient AMs essentially phenocopied those of b-catenin-deficient AMs, with more than 80% of differentially expressed genes in HIF-1a-deficient AMs overlapping with b-catenin-deficient AMs following Wnt3a treatment.

2021-05-05 | GSE164791 | GEO

Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes

Project description:To identify molecular characteristics of mouse AMs stimulated with or without Poly(I:C) in the presence of vehicle or MSDC overnight in vitro, we isolated RNA from AMs with various treatments and examined by bulk RNA-seq. We found a large number of cytokine gene profiles were reduced following MSDC treatment in WT AMs, suggesting that MSDC treatment recuced inflammatory response in AMs.

2023-12-02 | GSE216564 | GEO

Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes following DON treatment

Project description:To identify molecular characteristics of WT AM with or without DON stimulation in vitro, we isolated RNA from AMs with various treatments and examined by bulk RNA-seq. We found a large number of gene profiles were altered following DON treatment in WT AMs, suggesting that DON treatment altered the balance of proliferative versus inflammatory genes in AMs, and glutamine metabolism is important in regulating AM self-renewal ability.

2023-12-31 | GSE210076 | GEO

Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes and heterogeneity following Wnt3a treatment

Project description:Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes and heterogeneity following Wnt3a treatment

| PRJNA691955 | ENA

Bulk transcriptomic profiling of pluripotent stem cell-derived alveolar type 2 cells (iAT2s) cultured in spheres and at air-liquid interface and human HTII-280-sorted AT2s from a pediatric and adult donor lungs cultured in spheres

Project description:We performed bulk transcriptomic profiling of pluripotent stem cell-derived alveolar type 2 cells (iAT2s) and HTII-280-sorted human AT2s cultured with CK+DCI media. We used previously described distal lung directed differentiation protocol to generate alveolar type 2 cells (iAT2s). We used the SPC2-ST-B2 (“SPC2”) line containing a SFTPC-tdTomato knock in reporter. iAT2s were cultured in 3D spheres and at air-liquid interface. Human AT2s were HTII-280-sorted from a pediatric donor lung (13 months old) and an adult, nonsmoker human lung (32 years old) and cultured as spheres in the presence of MRC5s. After deconvolution, we find that iAT2s cultured in ALI correlate more closely to primary cultured AT2s than do iAT2s cultured in 3D spheres on the basis of lung epithelial gene expression.

2022-04-30 | GSE184143 | GEO

Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes and heterogeneity following Wnt3a treatment

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-05-05 | GSE164793 | GEO

Effect of estradiol on murine alveolar macrophages and RAW264.7 cells in vitro

Project description:Mouse BAL alveolar macrophages from pregnant E14 and control dams are compared to RAW cells cultured with estradiol to determine shared gene expression change patterns induced by pregnancy and estrogen BAL cells from intact pregnant and control mice were directly processed for RNA extraction. RAW cells were cultured overnight with estradiol or vehicle, then RNA was exctracted.

2014-01-01 | E-GEOD-52649 | biostudies-arrayexpress

Characterization of in vitro cultured alveolar macrophages (AM) heterogeneity following Wnt3a treatment [single-cell RNA-seq]

Project description:Characterization of in vitro cultured alveolar macrophages (AM) heterogeneity following Wnt3a treatment [single-cell RNA-seq]

| PRJNA691959 | ENA

Effect of estradiol on murine alveolar macrophages and RAW264.7 cells in vitro

2014-01-01 | GSE52649 | GEO

Bulk RNA sequencing of CSF1-cultured bone marrow-derived macrophages from WT and Clec4a2-/- mice.

Project description:CSF1-cultured macrophages were collected and sorted into CD11b+CD206high populations which then were separated into each genotype. Total RNA was extracted for Poly A bulk sequencing.

2021-10-28 | E-MTAB-10734 | biostudies-arrayexpress

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