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Transcriptome analysis of HCMV infected and mock-infected Kasumi-3 cells at 24 hours post infection

ABSTRACT: Transcriptome analysis of HCMV infected and mock-infected Kasumi-3 cells at 24 hours post infection

PROVIDER: PRJNA692191 | ENA |

REPOSITORIES: ENA

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Transcriptome analysis of HCMV infected and mock-infected Kasumi-3 cells at 24 hours post infection

Project description:Purpose: to compare host gene expression in HCMV infected and mock infected Kasumi-3 cells at 24 hours post infection

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Chromatin accessibilty analysis of HCMV infected and mock-infected Kasumi-3 cells at 24 hours post infection

Project description:Purpose: to compare host gene expression in HCMV infected and mock infected Kasumi-3 cells at 24 hours post infection and analyze viral chromatin accessibility

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Chromatin accessibilty analysis of HCMV infected and mock-infected Kasumi-3 cells at 24 hours post infection

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HCMV Latently infected Kasumi-3 transcription analysis.

Project description:HCMV latnecy within hematopoetic precursor cells was evaluated by infecting cells and establishing latency using a recombinant virus that allows for labeing of RNA species only in the context of a viral infection thereby allowing purification of these transcripts from mock infected cells. We used microarrays to detail the global programme of gene expression to identify changes in host transcription during HCMV latency.

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HCMV Latently infected Kasumi-3 transcription analysis.

Project description:HCMV Latently infected Kasumi-3 transcription analysis.

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Pol2 and H3K27Ac and H3K27me3 occupancy of the viral genome in HCMV infected Kasumi-3 cells at early times post infection

Project description:Purpose: to compare Pol2 and H3K27Ac and H3K27me3 in HCMV infected Kasumi-3 cells at 4 and 24 hours post infection

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CircRNA preofiles in mock-infected and human cytomegalovirus (HCMV) infected human embryonic lung fibroblasts (HELF) cells

Project description:In this study, RNA-seq analysis was performed to generally understand the circRNA profiles of mock-infected and HCMV-infected HELF cells. Totally, 27,409 and 35,515 host circRNAs were identified in mock-infected and HCMV-infected HELF cells by RNA-seq respectively. Among them, 278 circRNAs were significantly modified filtrating by a threshold value of >2 (or <-2) fold-change and q-value <0.05. GO and KEGG pathway enrichment analysis suggested that the remarkably changed circRNAs might play important roles in viral entry, cell proliferation, and inhibition of apoptosis. Verification of four selected circRNAs ( circSP100, circMAP3K1, circTRIO, and circPLEKHM1) was performed using RT-PCR and Sanger sequencing. Moreover, further verification of circSP100 was performed using northern blotting and RT-qPCR and proteins binding directly to circSP100 were purified using RNA antisense purification (RAP).

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