Burkholderia cenocepacia
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ABSTRACT:
PROVIDER: PRJNA694364 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Burkholderia cenocepacia sequence type 32 (ST32) represents one of the most globally distributed strains from Bukrholderia cepacia complex (Bcc), which infected 30% of Czech cystic fibrosis (CF) patients. The aim of this study was to compare gene expression in two pairs of ST32 clinical isolates that were subjected to cultivation in two different conditions, characteristic for chronic B. cenocepacia infection in CF patients. ST32 strain is known to be a problematic epidemic strain, which caused a serious outbreak at the Prague CF centre.
2015-02-27 | E-MTAB-3133 | biostudies-arrayexpress
Project description:Burkholderia cepacia complex (Bcc) comprises opportunistic bacteria infecting hosts such as cystic fibrosis (CF) patients. Bcc long-term infection of CF patient airways has been associated with emergence of phenotypic variation. Here we studied two Burkholderia multivorans clonal isolates (D2095 and D2214) displaying different morphotypes from a chronically infected CF patient in order to evaluate traits development during lung infection.
2011-08-22 | GSE28306 | GEO
Project description:Burkholderia cepacia complex (Bcc) comprises opportunistic bacteria infecting hosts such as cystic fibrosis (CF) patients. Bcc long-term infection of CF patient airways has been associated with emergence of phenotypic variation. Here we studied two Burkholderia multivorans clonal isolates (D2095 and D2214) displaying different morphotypes from a chronically infected CF patient in order to evaluate traits development during lung infection. Since the custom array described in platform GPL13356 was based on Burkholderia multivorans ATCC 17616 genome, here we performed a DNA-DNA hybridization to determine which probes of the array hybridize with our test genomes
2011-08-22 | GSE30402 | GEO
Project description:Transcriptional profiling of RNA-seq data from two Burkholderia species grown under conditions mimicking the cystic fibrosis lung and the soil environment
2009-11-21 | GSE19115 | GEO
Project description:In this work the genomic expression of two Burkholderia cenocepacia clonal variants (IST439 and IST4113) were compared. These isolates were collected from a chronically colonized cystic-fibrosis patient that has died from the cepacia syndrome. IST439 was the first B. cenocepacia isolate recovered from the patient and it is thought to have initiated the infection while the IST4113 isolate was recovered three years later, after a period of exacerbated infection that compelled the patient to hospitalization and intravenous therapy with gentamicine and ceftazidime. Among other phenotypic differences, IST4113 is much more resistant to a wide range of antimicrobials tested with very different biological targets
2012-08-01 | E-MTAB-809 | biostudies-arrayexpress
Project description:Burkholderia cepacia complex (Bcc) comprises opportunistic bacteria infecting hosts such as cystic fibrosis (CF) patients. Bcc long-term infection of CF patient airways has been associated with emergence of phenotypic variation. Here we studied two Burkholderia multivorans clonal isolates (D2095 and D2214) displaying different morphotypes from a chronically infected CF patient in order to evaluate traits development during lung infection. Since the custom array described in platform GPL13356 was based on Burkholderia multivorans ATCC 17616 genome, here we performed a DNA-DNA hybridization to determine which probes of the array hybridize with our test genomes Total DNA was extracted from overnight cultures of the mucoid D2095 and the nonmucoid D2214 isolates grown in LB medium.
2011-08-21 | E-GEOD-30402 | biostudies-arrayexpress
Project description:Hfq proteins are RNA chaperones that play a critical role in post-transcription regulation of gene expression. Bacteria of the Burkholderia cepacia complex harbor two distinct and functional Hfq proteins, the Hfq and Hfq2. We have previously performed the functional analysis of Hfq and Hfq2 in the pathogen Burkholderia cenocepacia J2315. In order to examine the impacts of each RNA chaperone on the global transcriptome of B. cenocepacia J2315, we performed comparative transcriptome profile of mutants on the hfq and hfq2 genes, using as reference the wild-type strain.
2014-01-01 | GSE47344 | GEO
Project description:Burkholderia cepacia complex (Bcc) comprises opportunistic bacteria infecting hosts such as cystic fibrosis (CF) patients. Bcc long-term infection of CF patient airways has been associated with emergence of phenotypic variation. Here we studied two Burkholderia multivorans clonal isolates (D2095 and D2214) displaying different morphotypes from a chronically infected CF patient in order to evaluate traits development during lung infection. For expression profiling, overnight cultures of the mucoid D2095 and the nonmucoid D2214 isolates grown in SM medium were diluted to an initial OD640 nm of 0.1 into the same medium. Triplicate samples were cultured at 37ºC with 250 r.p.m. agitation for 17 h and RNA extracted from the two bacterial isolates.
2011-08-21 | E-GEOD-28306 | biostudies-arrayexpress
Project description:Genomovar III isolate of cepacia complex that can opportunistically infect cystic fibrosis patients
| PRJNA13918 | ENA
Project description:Genomovar III isolate of cepacia complex that can opportunistically infect cystic fibrosis patients
| PRJNA16169 | ENA