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An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation

ABSTRACT: An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation

PROVIDER: PRJNA694981 | ENA |

REPOSITORIES: ENA

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An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation (smallRNA-seq dataset)

Project description:An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation (smallRNA-seq dataset)

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Project description:An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation (BSA-seq dataset)

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An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation

Project description:This SuperSeries is composed of the SubSeries listed below.

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An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation (smallRNA-seq dataset)

Project description:Foreign DNA is silenced in plants by small RNA-directed DNA methylation (RdDM). RNA Polymerase V (Pol V) is the key protein required to recruit DNA methyltransferases to a RdDM target region. However, the mechanism required for the initial recruitment of Pol V to a targeted locus remains a mystery. In this study, we used first generation (T1) transgene transformations to elucidate a novel mechanism by which Pol V is recruited to both new transgenic and endogenous loci. This study is heavily dependent on analyzing the DNA methylation pattern and small RNAs produced from specific loci. All small RNA sequences (sRNA-seq) and bisulfite converted amplicon sequencing data (BSAS) used in this study are submitted as part of this data series.

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Project description:During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of all Ago protein complexes expressed in diverse species to identify associated proteins, small RNAs or target mRNAs. We refer to our method as Ago protein Affinity Purification by Peptides (Ago-APP). Comparison of small RNA lengths in total RNA and APP-enriched RNA samples

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Project description:AGO pathway

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Project description: Not available

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