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Omics score: 1000

An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation

ABSTRACT: An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation

PROVIDER: PRJNA694981 | ENA |

REPOSITORIES: ENA

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An siRNA-guided ARGONAUTE protein directs RNA polymerase V to initiate DNA methylation.

Sigman Meredith J MJ Panda Kaushik K Kirchner Rachel R McLain Lauren L LL Payne Hayden H Peasari John Reddy JR Husbands Aman Y AY Slotkin R Keith RK McCue Andrea D AD

Nature plants 20211108 11

In mammals and plants, cytosine DNA methylation is essential for the epigenetic repression of transposable elements and foreign DNA. In plants, DNA methylation is guided by small interfering RNAs (siRNAs) in a self-reinforcing cycle termed RNA-directed DNA methylation (RdDM). RdDM requires the specialized RNA polymerase V (Pol V), and the key unanswered question is how Pol V is first recruited to new target sites without pre-existing DNA methylation. We find that Pol V follows and is dependent o ...[more]

PMID: 34750500

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An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation (BSA-seq dataset)

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