Project description:hybridisations using 1.04 Arabidopsis thaliana - columbia-0 and landsberg plants:plants cultivated and RNA-extracted by VIB, amplified and hybridised at URGV. aVIB-Ler_vs_aVIB-Col
Project description:Four-week-old Arabidopsis thaliana (Col-0) plants were fumigated for 1 h with 10 parts per million (ppm) nitrogen dioxide (NO2) to analyse leaf transcriptome changes induced by this air pollutant relative to air-fumigated control plants.
Project description:Autopolyploidy is a process whereby the chromosome set is multiplied and it is a common phenomenon in angiosperms. Autopolyploidy is thought to be an important evolutionary force that has led to the formation of new plant species. Despite its relevance, the consequences of autopolyploidy in plant metabolism are poorly understood. This study compares the metabolic profiles of natural diploids and artificial autotetraploids of Arabidopsis thaliana Col-0. Different physiological parameters are compared between diploids and autotetraploids using nuclear magnetic resonance (NMR), elemental analysis (carbon:nitrogen balance) and quantitative real-time PCR (qRT-PCR). The main difference between diploid and autotetraploid A. thaliana Col-0 is observed in the concentration of metabolites related to the tricarboxylic acid cycle (TCA) and ?-amino butyric acid (GABA) shunt, as shown by multivariate statistical analysis of NMR spectra. qRT-PCR shows that genes related to the TCA and GABA shunt are also differentially expressed between diploids and autotetraploids following similar trends as their corresponding metabolites. Solid evidence is presented to demonstrate that autopolyploidy influences core plant metabolic processes.
