Project description:Transcriptional profiling to identify genes differentially regulated by Sufu during cerebellum development. Sufu was specifically deleted from the granule neuron compartment using Math1-driven Cre recombinase.
Project description:Sepsis is a potentially life-threatening condition caused by the body's response to a severe infection. However, the pathogenesis of sepsis remains unclear. Here, we found that Suppressor of Fused (Sufu), a classical negative regulator of Hedgehog signaling, is downregulated in patients with sepsis and in murine peritoneal macrophages treated with lipopolysaccharide (LPS). Deletion of Sufu aggravated LPS-induced lung injury and lethality in mice, and augmented LPS induced proinflammatory gene expression in cultured macrophages. By RNA-seq transcriptome profiling, we identified Sufu as a negative regulator of the TLR-triggered inflammatory response.
Project description:SUFU is a negative regulator of the hedgehog signaling pathway and its abnormal expression has been found in several human cancers, whereas the role of SUFU in the malignant development of breast cancer has not been fully elucidated. We performed RNA-seq to determine the impact of SUFU overexpression and knockdown on global gene expression profile in SKBR3 and HCC1954 cells. Our results show that sufu affects ferroptosis phenotype via modulating yap-dependent ACSL4 in breast cancer cells.
