Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes [Quant-seq]

ABSTRACT: Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes [Quant-seq]

PROVIDER: PRJNA699852 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes [Quant-seq]

Project description:Direct lineage conversion holds great promise in the regenerative medicine field for restoring damaged tissues using functionally engineered counterparts. However, current methods of direct lineage conversion, even those employing virus-mediated transgenic expression of tumorigenic factors, are extremely inefficient (~25%). Thus, advanced methodologies capable of revolutionizing efficiency and addressing safety issues are key to clinical translation of these technologies. Here, we propose an exosome-guided, non-viral, direct-lineage conversion strategy to enhance transdifferentiation of fibroblasts to induced cardiomyocyte-like cells (iCMs). Exosomes produced during the cardiac differentiation process of embryonic stem cells (ESCs) are able to achieve extremely high reprogramming efficiency (>60%) by generating functional iCMs from mouse embryonic fibroblasts via a cardiac precursor-like stage rather than a pluripotent state. The resulting iCMs possess typical cardiac Ca2+ transients and electrophysiological features, and exhibit global gene expression profiles similar to those of cardiomyocytes. The optimized reprogramming conditions produce beating iCM clusters ~3-fold more efficiently than conventional methods. This is the first demonstration of the use of exosomes derived from ESCs undergoing cardiac differentiation as biomimetic tools to induce direct cardiac reprogramming with greatly improved efficiency, establishing a general, more readily accessible platform for broadly generating a variety of specialized somatic cells through direct lineage conversion.

2022-03-02 | GSE166249 | GEO

Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes

Project description:Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes

| PRJNA699851 | ENA

Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes [miRNA-Seq]

Project description:Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes [miRNA-Seq]

| PRJNA699853 | ENA

Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes [miRNA-Seq]

2022-03-02 | GSE166250 | GEO

Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-03-02 | GSE166251 | GEO

Ultrasound-mediated ultra effective cellular reprogramming of human fibroblasts into neural progenitor-like cells [MBD-Seq]

Project description:We have developed an ultra effective method of triggering fast cellular reprogramming into neural progenitor cells (NPCs) with significant yields, called ultrasound-directed permeation of environmental transition-guided cellular reprogramming (Entr).

2019-09-20 | GSE87204 | GEO

Ultrasound-mediated ultra effective cellular reprogramming of human fibroblasts into neural progenitor-like cells [RNA-Seq]

2019-09-20 | GSE86644 | GEO

An ultra effective method of generating extra multipotent cells from human fibroblasts via ultrasound-directed instant chromatin remodeling

Project description:We have developed an ultra effective method of generating extra multipotent (xpotent) cells which have differentiation potential more than limited cell types, by the mechanism of ultrasound-directed permeation of environmental transition-guided cellular reprogramming (Entr).

2019-09-09 | GSE86646 | GEO

An ultra effective method of generating extra multipotent cells from human fibroblasts via ultrasound-directed instant chromatin remodeling [MBD-seq]

2019-09-09 | GSE86647 | GEO

Nuclear exosome

Project description:Nuclear exosome

| PRJNA304634 | ENA