Project description:Chromatin state profiling using multiple histone modifications in isolated wild type E13.5 mandibular mouse incisors. Imputed files were generated using these files and data obtained from ENCODE embryonic and postnatal mouse ChIP samples.
Project description:By using His6-tagged recombinant human secretagogin in the presence of Ca2+ (100 µM), we isolated its putative interacting partners from mouse embryonic (E13.5) pancreata and identified their amino acid sequences by mass spectrometry. Next to previously described interacting partners (participating in vesicle-mediated transport, cytoskeletal organization and members of chaperonin-containing complex), we found a substantial group of proteins involved in proteasomal catabolic process including 15 different subunits of the 26S proteasome. Our data support that secretagogin protects Pdx1 from proteasomal degradation to control a transcriptional program required for β cell specification.
Project description:In this study we performed single-cell sequencing of E13.5 mouse cerebella, revealing different newly generated neurons and their associated molecular features.
Project description:Experiment to determine the genome-wide distribution of P63 binding regions, using an antibody specific to the alpha sub-unit, in mouse (E13.5/E14.5) secondary palatal shelf tissue.