Project description:mRNA expression data for VPS39-silenced human muscle cells and controls (n=6 per group) were obtained using GeneChip™ Human Gene 2.0 ST Assay (Applied Biosystems)
Project description:African swine fever virus (ASFV) is a large DNA virus causing a highly contagious disease in domestic and wild boar, for which no treatment is available. ASFV vaccine development is hindered by large gaps in knowledge considering virus protein function and virus-host interaction. This study shows that the ASFV CP204L is a multifunctional protein engaged in endosomal trafficking and localizes to virus replication sites. Moreover, VPS39, a component of the HOPS complex, is a direct host interactor of CP204L. The virus CP204L binds the VPS39 domain responsible for its recruitment to the endosomal membrane, prevents its integration into the HOPS complex, and promotes the clustering of lysosomes. Additionally, we show that VPS39 is an important factor in the early phase of infection, as virus replication and protein synthesis in VPS39 knockout cells are delayed. These results uncover a novel function of viral protein CP204L and extend our understanding of complex interaction between virus and host, providing insights for developing a vaccine to prevent and control ASFV.
Project description:mRNA expression data for the mouse muscle (n=6 per sex and genotype, for a total of 24 mice) were obtained using Clariom™ S Assay Mouse (Applied Biosystems) according to the manufacturer’s recommendations.
Project description:To determine the potential mechanisms by which AID-elimination facilitate better J558 tumor rejection by P1CTL, we performed cDNA microarray analysis to compare AID-silenced J558 cells and their relative controls.
Project description:The 1q gain is related to poor survival, and to a profile of cell cycle deregulation in Ewing's Sarcoma (ES). Tumor samples with 1q gain overexpress the gene DTL. We have silenced DTL in three ES cell lines in order to compare the differential expression pattern produced with the differential expression pattern which characterizes 1q gained-tumors. We silenced DTL in three ES cell lines with two different shRNA constructions by means of lentiviral transduction. pLKO.1-non-targeting control was transduced in the control samples. RNA was extracted and hybridized with Affymetrix expression microarrays.