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Modeling SARS-CoV-2 infection and its individual differences with ACE2-expressing human iPS cells

ABSTRACT: Modeling SARS-CoV-2 infection and its individual differences with ACE2-expressing human iPS cells

PROVIDER: PRJNA702533 | ENA |

REPOSITORIES: ENA

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Modeling SARS-CoV-2 infection and its individual differences with ACE2-expressing human iPS cells

Project description:Genetic differences are a primary reason for differences in the susceptibility and severity of COVID-19. As induced pluripotent stem (iPS) cells maintain the genetic information of the donor, they can be used to model individual differences in SARS-CoV-2 infection in vitro. We found that human iPS cells expressing the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) (ACE2-iPS cells) can be infected w SARS-CoV-2. In infected ACE2-iPS cells, the expression of SARS-CoV-2 nucleocapsid protein, budding of viral particles, and production of progeny virus, double membrane spherules, and double-membrane vesicles were confirmed. We performed SARS-CoV-2 infection experiments on ACE2-iPS/ embryonic stem (ES) cells from eight individuals. Male iPS/ES cells were more capable of producing the virus compared with female iPS/ES cells. These findings suggest that ACE2-iPS cells can not only reproduce individual differences in SARS-CoV-2 infection in vitro but also are a useful resource to clarify the causes of individual differences in COVID-19 due to genetic differences.

2021-04-18 | GSE166990 | GEO

Modeling SARS-CoV-2 infection and its individual differences with ACE2-expressing human iPS cells.

Project description: Not available

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Regulation by the homologous Ace2 and Swi5 factors of Saccharomyces cerevisiae

Project description:The homologous Ace2 and Swi5 transcription factors of Saccharomyces cerevisiae have identical DNA-binding domains, and both are cell cycle regulated. There are common target genes, as well as genes activated only by Ace2 and other genes activated only by Swi5. Keywords: genetic modification RNA was isolated from four strains: wild type, ace2 gene deletion, swi5 gene deletion, and the ace2 swi5 double gene deletion. RNAs from the three mutant strains were compared to wild type RNA in a microarray hybridization experiment.

2008-05-13 | E-GEOD-11380 | biostudies-arrayexpress

Effects of ACE2 on BMPR2 mutation-mediated defects in gene expression

Project description:BMPR2 mutation causes pulmonary arterial hypertension (PAH); ACE2 treatment can resolve established BMPR2-mediated PAH. The purpose of this study was to uncover the molecular mechanism behind this. Four groups: +/- ACE2 and +/- BMPR2 transgene, two arrays each, each array a pool of three animals.

2010-08-24 | E-GEOD-21583 | biostudies-arrayexpress

Modeling Cellular Crosstalk and Organotypic Vasculature Development with Human iPSC-Derived Endothelial Cells (iPS-EC) and Cardiomyocytes (iPS-CM) [scRNA-seq]

Project description:The aim was to study the effect of 1) coculture of iPS-ECs and iPS-CMs on each cell type and 2) shear stress on iPS-derived endothelial cells (iPS-EC). The transcriptional landscapes of each cell type were compared.

2020-11-06 | GSE150741 | GEO

Modeling SHH-driven medulloblastoma with patient iPS cell-derived neural stem cells

Project description:Modeling SHH-driven medulloblastoma with patient iPS cell-derived neural stem cells

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Disease modeling of pulmonary fibrosis using human iPS cell-derived alveolar organoids

Project description:Disease modeling of pulmonary fibrosis using human iPS cell-derived alveolar organoids

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Modeling Cellular Crosstalk and Organotypic Vasculature Development with Human iPSC-Derived Endothelial Cells (iPS-EC) and Cardiomyocytes (iPS-CM) [RNA-seq]

Project description:The aim was to study the effect of shear stress on iPS-derived endothelial cells (iPS-EC). iPS-ECs were grown in static conditions and in flow in the Ibidi Pump system. The transcriptional landscapes of cells grown in these 2 conditions were compared.

2020-11-06 | GSE150740 | GEO

Disease modeling of pulmonary fibrosis using human iPS cell-derived alveolar organoids (fibroblasts)

Project description:Disease modeling of pulmonary fibrosis using human iPS cell-derived alveolar organoids (fibroblasts)

| PRJNA722176 | ENA

Modeling Cellular Crosstalk and Organotypic Vasculature Development with Human iPSC-Derived Endothelial Cells (iPS-EC) and Cardiomyocytes (iPS-CM) [scRNA-seq]

Project description:Modeling Cellular Crosstalk and Organotypic Vasculature Development with Human iPSC-Derived Endothelial Cells (iPS-EC) and Cardiomyocytes (iPS-CM) [scRNA-seq]

| PRJNA633490 | ENA

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