Dynamic transcriptome and chromatin architecture in granulosa cells during chicken folliculogenesis
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ABSTRACT:
PROVIDER: PRJNA702811 | ENA |
REPOSITORIES: ENA
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Project description:Ovarian follicle development is a complex and well-orchestrated biological process. Granulosa cells (GCs) are the most important somatic cells in the ovary, which play important roles in the growth of follicles. Three-dimensional (3D) chromatin architecture has emerged as an important regulator of transcription in eukaryote, but how these changes in granulosa cells during folliculogenesis of chicken are largely unexplored. Here, we performed an integrative analysis of transcriptomic and chromatin structural characterization for chicken follicular granulosa cells of 10 and 3 growth stages respectively.
2021-11-10 | GSE181756 | GEO
Project description:Ovarian follicle development is a complex and well orchestrated biological process. Chromatin spatial organization has emerged as an important regulator of gene expression, but how these changes during follicle development are still unknown. Here, we integrated RNA-seq and Hi-C analyses of chicken follicular granulosa cells of 10 developmental stages. Our data revealed that SWF, F1, and POF had the greatest transcriptome differences from other stages. Our results provide a genome-wide atlas of chromatin interactions during chicken ovarian follicle development.
2021-11-10 | GSE167064 | GEO
Project description: Not available
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Project description:The dynamic transcriptional regulation and interactions of human germlines and surrounding somatic cells during folliculogenesis remains unknown. Using RNA-Seq analysis of human oocytes and corresponding granulosa cells (GCs) spanning five follicular stages, we revealed unique features in transcriptional machinery, transcription factor networks and reciprocal interactions in human oocytes and GCs that displayed developmental-stage-specific expression patterns. Notably, we identified specific gene signatures of two cell types in particular developmental stage that may reflect developmental competency and ovarian reserve. Additionally, we uncovered key pathways that may concert germline-somatic interactions and drive the transition of primordial-to-primary follicle which represents follicle activation. Thus, our work provides key insights into the crucial features of the transcriptional regulation in the stepwise folliculogenesis and offers important clues for improving follicle recruitment in-vivo and restoring fully competent oocytes in-vitro.
2018-11-20 | GSE107746 | GEO
Project description:Transcriptomics of granulosa cells from different developing stages (6mm, F5, F1) in chicken ovary
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Project description:Transcriptomics of proximal granulosa cells vs distal granulosa cells from F5 and F1 in chicken ovary
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Project description:Transcriptome Landscape of Human Folliculogenesis Reveals Oocytes and Granulosa Cells Interactions
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Project description:In mammalian ovaries, granulosa cells (GCs) behave as the most active cell lineage in terms of differentiation and proliferation, ensuring precise developmental gene expression pattern and enhancing continuous ovarian folliculogenesis; however, how developing GCs is interplay with downstream transcriptional activation in mammals remain poorly understood. In this research, we depicted the genome-wide redistribution of accessible chromatin regions that lead to broad developmental-related transcriptome effects spanning pig and mouse follicular stages. We report the distinct GCs-activated accessibility regions (GAAs) at MII stage are always distributed on H3K4me3-anchored promoter regions, where they are responsible for elevated flanking development related genes (DRGs) expression, serving as a key requirement for transcription in response to developmental signals. Mechanistically, the transcription factor, Fosl2, can be mightily recruited to GAAs to accomplish chromatin accessibility state transition. The enlarged GAAs signal driven by Fosl2 loading subsequently induces a robust increase in the expression of adjacent DRGs expression, which contribute to the progression of ovarian folliculogenesis. Together, our study delineates the dynamic chromatin accessibility landscape across various stages of folliculogenesis, uncovering a pivotal role for the Fosl2 in controlling the transcriptional activation through the reconfiguration of the widespread chromatin state.
2024-06-01 | GSE267851 | GEO
Project description:In mammalian ovaries, granulosa cells (GCs) behave as the most active cell lineage in terms of differentiation and proliferation, ensuring precise developmental gene expression pattern and enhancing continuous ovarian folliculogenesis; however, how developing GCs is interplay with downstream transcriptional activation in mammals remain poorly understood. In this research, we depicted the genome-wide redistribution of accessible chromatin regions that lead to broad developmental-related transcriptome effects spanning pig and mouse follicular stages. We report the distinct GCs-activated accessibility regions (GAAs) at MII stage are always distributed on H3K4me3-anchored promoter regions, where they are responsible for elevated flanking development related genes (DRGs) expression, serving as a key requirement for transcription in response to developmental signals. Mechanistically, the transcription factor, Fosl2, can be mightily recruited to GAAs to accomplish chromatin accessibility state transition. The enlarged GAAs signal driven by Fosl2 loading subsequently induces a robust increase in the expression of adjacent DRGs expression, which contribute to the progression of ovarian folliculogenesis. Together, our study delineates the dynamic chromatin accessibility landscape across various stages of folliculogenesis, uncovering a pivotal role for the Fosl2 in controlling the transcriptional activation through the reconfiguration of the widespread chromatin state.
2024-06-01 | GSE267850 | GEO
Project description:In mammalian ovaries, granulosa cells (GCs) behave as the most active cell lineage in terms of differentiation and proliferation, ensuring precise developmental gene expression pattern and enhancing continuous ovarian folliculogenesis; however, how developing GCs is interplay with downstream transcriptional activation in mammals remain poorly understood. In this research, we depicted the genome-wide redistribution of accessible chromatin regions that lead to broad developmental-related transcriptome effects spanning pig and mouse follicular stages. We report the distinct GCs-activated accessibility regions (GAAs) at MII stage are always distributed on H3K4me3-anchored promoter regions, where they are responsible for elevated flanking development related genes (DRGs) expression, serving as a key requirement for transcription in response to developmental signals. Mechanistically, the transcription factor, Fosl2, can be mightily recruited to GAAs to accomplish chromatin accessibility state transition. The enlarged GAAs signal driven by Fosl2 loading subsequently induces a robust increase in the expression of adjacent DRGs expression, which contribute to the progression of ovarian folliculogenesis. Together, our study delineates the dynamic chromatin accessibility landscape across various stages of folliculogenesis, uncovering a pivotal role for the Fosl2 in controlling the transcriptional activation through the reconfiguration of the widespread chromatin state.
2024-06-01 | GSE267849 | GEO