Gene expression levels of evolved thermotolerant yeast and its parent under fermentation condition
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ABSTRACT:
PROVIDER: PRJNA703366 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Purpose: To explore the differentially expressed genes and pathways in the thermotolerant mutant compare to wildtype. To figure out the contribution of these genes and pathways to high protein expression ability in the mutant. Method: the samples were collected from the 60h fermentation culture. The fermentation medium was BMGY/BMMY with 1%(v/v) methanol as inducer. the cultivation condition was set under 28℃ and 37℃. Results: after data analysis, we found genes related to oxidative stress response were important.
2021-07-28 | GSE167141 | GEO
Project description:Gene expression levels of evolved thermotolerant yeast and its parent
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Project description:Samples for RNA-seq were collected from cell cultures in YPD medium under 30 °C or 37 °C at the logarithmic phase. The gene expression levels of thermotlerant yeast and the parent under these two temperatures were analyzed. We found the most upregualted genes of the parent under high temperature versus normal temperature were involved in autophay. the peroxisome pathway was significantly upregulated in G14 under high temperature versus the parent under normal temperature. The antixidant system of yeast was activated under thermal stress.
2021-07-20 | GSE157242 | GEO
Project description:Gene expression in response to severe heat stress (50°C 1 h) was compared between thermotolerant and thermosensitive tomato cultivars. The thermosensitive cultivar showed severe impairment of heat stress response at this condition, while the thermotolerant cultivar were less affected. The results of this experiment were used to select expression marker genes that reflect the cellular heat shock response and correlate with thermotolerance.
2023-12-30 | E-MTAB-12094 | biostudies-arrayexpress
Project description:Gene expression in response to heat stress (40°C 2 h + 50°C 4 h) was compared between thermotolerant and thermosensitive tomato cultivars. Under this condition, the thermosensitive cultivar showed severe shoot wilting, while the themotolerant cultivar did not.
2023-12-30 | E-MTAB-12093 | biostudies-arrayexpress
Project description:Chemostat evolution experiment C1 under glucose-limited condition at 30C. Gene Expression profiles of adaptive clones M1-M5 in evolved conditions compared with original parents. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Genotype: Evolved clones. The genotype is not necessarily complete. Keywords: all_pairs
2008-08-20 | GSE11071 | GEO
Project description:Chemostat evolution experiment C1 under glucose-limited condition at 30C. Gene Expression profiles of adaptive clones M1-M5 in evolved conditions compared with original parents. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Genotype: Evolved clones. The genotype is not necessarily complete. Keywords: all_pairs Gene Expression profiles of adaptive clones M1-M5 in evolved conditions compared with original parents
2008-08-20 | E-GEOD-11071 | biostudies-arrayexpress
Project description:A population of Saccharomyces cerevisiae was cultured for approximately 450 generations in the presence of high glucose to select for genetic variants. This experiment allows for a controlled model of adaptive evolution under natural selection. Using the parental strain BY4741 as the starting population, an evolved culture was obtained after continuous aerobic growth in a glucose-high medium for approximately 450 generations. After the evolution period three single colony isolates were selected for analysis. Next-generation Ion Torrent sequencing was used to evaluate genetic changes. Greater than 100 deletion/insertion changes were found with approximately half of these effecting genes. Additionally, over 180 single-nucleotide polymorphisms (SNPs) were identified with more than one quarter of these resulting in a non-synonymous mutation. Affymetrix DNA microarrays and RNseq analysis were used to determine differences in gene expression in the evolved strains compared to the parental strain. It was established that approximately 900 genes demonstrated significantly altered expression in the evolved strains relative to the parental strain. Many of these genes showed similar alterations in their expression in all three evolved strains. Interestingly, genes with altered expression in the three evolved strains included genes with a role in the TCA cycle. Overall these results are consistent with the physiological observations of decreased ethanol production and suggest that the underlying metabolism switched from fermentation to respiration during aerobic growth. Yeast strains were grown in the presence of high glucose for approximately 450 generations. Individual isolates were then grown and total RNA was collected to compare difference between the evolved strains and the parental strain.
2012-07-10 | E-GEOD-36518 | biostudies-arrayexpress
Project description:A population of Saccharomyces cerevisiae was cultured for approximately 450 generations in the presence of high glucose to select for genetic variants. This experiment allows for a controlled model of adaptive evolution under natural selection. Using the parental strain BY4741 as the starting population, an evolved culture was obtained after continuous aerobic growth in a glucose-high medium for approximately 450 generations. After the evolution period three single colony isolates were selected for analysis. Next-generation Ion Torrent sequencing was used to evaluate genetic changes. Greater than 100 deletion/insertion changes were found with approximately half of these effecting genes. Additionally, over 180 single-nucleotide polymorphisms (SNPs) were identified with more than one quarter of these resulting in a non-synonymous mutation. Affymetrix DNA microarrays and RNseq analysis were used to determine differences in gene expression in the evolved strains compared to the parental strain. It was established that approximately 900 genes demonstrated significantly altered expression in the evolved strains relative to the parental strain. Many of these genes showed similar alterations in their expression in all three evolved strains. Interestingly, genes with altered expression in the three evolved strains included genes with a role in the TCA cycle. Overall these results are consistent with the physiological observations of decreased ethanol production and suggest that the underlying metabolism switched from fermentation to respiration during aerobic growth.
2012-07-11 | GSE36518 | GEO
Project description:RNA-seq was performed on T. maritima wild type, three glucose evolved cultures, and three glycerol adapted cultures. Wild type and glucose evolved strains were grown on glucose minimal media and glycerol evolved cultures were grown on glycerol minimal media. All samples were harvested in exponential phase. Seven samples are included with two biological replicates for each condition.
2015-06-09 | E-GEOD-63141 | biostudies-arrayexpress