Project description:CRISPR knock out the Satb2 or Foxd2 gene from mouse colonic organoids to validate core human and mouse large intestine stem cell enrich TFs.
Project description:Resequening ileum and colon epithelium with deeper reads to evaluate consequence of Satb2 gene loss in the colonic epithelium. The same library construction method was used to compare with Bulk RNA-seq data from In Vitro cultured mouse organoids.
Project description:Given that SATB2 is expressed in differentiated colonic cells as well as the stem cells, we evaluated whether SATB2 might directly regulate the identity of the differentiated cells
Project description:We use ChIP-seq to understand the genomic binding sites of SATB2 in colonic epithelium and to study the shifting of CDX2 and HNF4A's genomic binding sites after SATB2 loss in colonic epithelium.
Project description:To investigate if Satb2 is sufficient to confer colonic fate from the small intestine mucosa, we generated a transgenic mouse line, CAGSATB2-GFP, in which CRE excision of a stop cassette activates HA epitope-tagged SATB2 and GFP fluorescence.