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Single cell RNA-sequencing in the suprafornical area of lateral hypothalamus

ABSTRACT: Single cell RNA-sequencing in the suprafornical area of lateral hypothalamus

PROVIDER: PRJNA704080 | ENA |

REPOSITORIES: ENA

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Single cell RNA-sequencing in the suprafornical area of lateral hypothalamus

Project description:The single-cell RNA sequencing was focused on the suprafornical LHA and its surrounding areas. For single cell dissociation and collection, we used transgenic animal Agrp-IRES-Cre × Ai9 crosses, where the tdTomato-labeled AGRP neurons extend a prominent set of axonal projections to the suprafornical region of the LHA and provide a useful signal for visually guided dissection of the targeting brain region. Single-cell cDNA libraries were prepared using the Smart-SCRB chemistry.

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