Project description:We prepared spinal cords from Optn floxed and KO mice treated with vehicle or the RIPK1 inhibitor Nec-1s, which were enriched for CD45+ cells and single cell RNA sequenced using the inDrop protocol.
Project description:T cells are considered to be an important cell type for pathogenesis of multiple screlosis. We used single cell RNA sequencing (scRNA-seq) to analyze popluation of inflitrating CD45+ cells in spinal cords of WT and STAP-1 KO mice to figure out whehter STAP-1 is required for infiltration of T cells in the spinal cords after EAE induction.
Project description:We performed scRNAseq analysis of CD45- cells sorted from murine brains on the presence or absence of a fluorescent reporter for complement component C3 at peak of experimental autoimmune encephalomyelitis to study the non-immune cellular sources of C3
Project description:T cells are considered to be an important cell type for pathogenesis of multiple sclerosis. We used single cell RNA sequencing (scRNA-seq) to analyze popluation of inflitrating CD45+ cells in spinal cords of newly developed inhibitor-treated and untreated mice to figure out whehter the inhibitor can suppress severity of EAE by inhibiting infiltration of T cells in the spinal cords.
Project description:Exploration of proteome differences between CD45+ and CD45- cell types in renal cell carcinoma tumors and normal adjacent tissue patient samples.