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Genome-wide maps in MDA-MB-231 cells treated with or without CPUL119(4.5 μM), CPUL129(1.8 μM), CPUL149(0.9 μM)

ABSTRACT: Genome-wide maps in MDA-MB-231 cells treated with or without CPUL119(4.5 μM), CPUL129(1.8 μM), CPUL149(0.9 μM)

PROVIDER: PRJNA706417 | ENA |

REPOSITORIES: ENA

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Genome-wide maps in MDA-MB-231 cells treated with or without CPUL119(4.5 μM), CPUL129(1.8 μM), CPUL149(0.9 μM)

Project description:Analysis of MDA-MB-231 cells following CPUL119, CPUL129, CPUL149 treatment or not. CPUL119, CPUL129, CPUL149 regulates various mRNA expression in MDA-MB-231 cells.Results provide insight into the role of mRNAs-involved mechanisms underlying CPUL119-, CPUL129-, CPUL149-mediated effects on breast cancer stemness.

2022-03-01 | GSE168188 | GEO

Transcriptomic profiling of MDA-MB-231 cells treated with TNFɑ [40 ng/ml] ± Apigenin [40 μM].

Project description:The effects of TNFɑ [40 ng/ml] ± Apigenin [40 μM] were evaluated in MDA-MB-231 cells using GeneChip™ Human Gene 2.1 ST Arrays by Affymetrix Inc assessing for mRNAs and long intergenic non-coding RNA transcripts.

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Gene expression profiles of MDA-MB-231 breast cancer cell line treated with DMSO, 10 μM DAC, 1 μM DEX, or DAC+DEX for 6 days.

Project description:We observed gene expression difference between different groups after MDA-MB-231 treated with DMSO, 10 μM DAC, 1 μM DEX, or DAC+DEX. Data obtained from high-throughput sequencing (Illumina NovaSeq 6000 platform) were transformed into raw sequenced reads by CASAVA base calling and stored in FASTQ format. Gene expression of each groups are listed in raw data files. Some different expression genes between two groups are further validated with qRT-PCR.

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IGFBP6 knokdown in MDA-MB-231 cell line

Project description:Breast cancer line MDA-MB-231 was cultured in complete culture medium containing high glucose DMEM (4.5 g/L) supplemented with 10% fetal bovine serum, 2 mM L-glutamine and 1% antibiotic-antimycotic solution ( Gibco) in an MCO-18AC incubator (Sanyo, Japan) at 37ºC and 5% CO2 concentration in the air. Cell reseeding was carried out every 2-3 days according to the standard protocol. The protocol for obtaining the MDA-MB-231 cell line with stable knockdown of IGFBP6 was described previously (Nikulin et al., 2021).

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The miRNA profiling in MDA-MB-231, MDA-MB-231/E1A and knockdown of Dicer in MDA-MB-231/E1A cells

Project description:Dicer, RNase III endonuclease, is an essential enzyme in miRNA biogenesis that regulates target gene expression, and it has been reported that aberrant expressions of Dicer associate with the clinical outcomes of patients in various cancers. To explore the miRNA differencial expression regulated by Dicer in MDA-MB-231/E1A cells, the microarray profiling analysis was employed to conduct differentially expressed miRNAs in stable MDA-MB-231/vector, MDA-MB-231/E1A, and MDA-MB-231/E1A/shDicer cells.

2016-01-16 | GSE76924 | GEO

The miRNA profiling in MDA-MB-231, MDA-MB-231/E1A and knockdown of Dicer in MDA-MB-231/E1A cells

2016-01-16 | E-GEOD-76924 | biostudies-arrayexpress

Genome-wide maps in MDA-MB-231 cells treated with or without HET0016 (5 μM).

Project description:Analysis of MDA-MB-231 cells following HET0016 treatment or not. HET0016 regulates various mRNA expression in MDA-MB-231 cells.Results provide insight into the role of mRNAs-involved mechanisms underlying HET0016-mediated effects on breast cancer stemness.

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Expression profiles of MDA-MB-231, MDA-231 S1a and S1b

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Gene expression profiles of NR1D1 knockout MDA-MB-231 cells treated with H2O2

Project description:To understand the role of NR1D1 in the progression of breast cancer, NR1D1 knockout (KO) MDA-MB-231 cells, established using the CRISPR/Cas9 system, were treated with 200 μM H2O2 for 72 h. Gene expression patterns were analyzed by microarray.

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Expression profiles of MDA-MB-231, MDA-231 S1a and S1b

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