Project description:To investigate the differential expression of lncRNAs and mRNAs, and even their biological functions in human proximal tubular epithelial (HK-2) cells induced by either normal glucose or high levels of glucose. Keywords: Expression profiling by array
Project description:To investigate the differential expressed circRNAs, and even their biological functions in human proximal tubular epithelial (HK-2) cells induced by normal glucose, high levels of glucose and treatment of dapagliflozin, metformin and vildagliptin.
Project description:We observe dyseregulated CD200-CD200R signaling in early diabetes that correlates with microglia-mediated neuroretinopathy and found that CD200 fusion protein (CD200Fc), an agonist of CD200R, attenuated high glucose-induced inflammation in cultured microglia. Therefore, we performed RNA-Sequencing on cultured BV2 microglia following exposure to normal or high glucose media (NG, HG) with or without CD200Fc supplementation (50ng/mL) to investigate broader biologic implications.
Project description:We first performed miRNA analysis on SU-4 cells in suspension, and after 24 and 48 h of HK co-culture. To ensure that no HK cell contamination occurred, SU-4 cells in suspension and after co-culture were purified by CD19-positive magnetic selection, followed by total RNA isolation. Two-condition experiment, S4 vs. S4+HK cells. Biological replicates: 2 suspension controls, 2 co-cultured with HK cells, independently grown and harvested. One replicate per array.
Project description:Transcriptional profiling of Mouse cavernous primary pericytes (MCPs) in comparing normal glucose condition with high glucose condition
Project description:We report that high glucose can have a detrimental effect on the stability of TET2 protein, and thereby reduce the genome-wide 5hmC content. We use MeDIP and hMeDIP followed by sequencing to identify the change of 5hmC and 5mC landscapes in A2058-TET2WT over expressing cells cultured in high and normal glucose concentrations.
Project description:Primary isolated rat dorsal root ganglion nerve cells (DRGs) were cultured with high glucose in vitro and divided into normal culture group and high glucose culture group
Project description:The aim of the present work was to study the effects of benfotiamine (S-benzoylthiamine O-monophosphate) upon glucose and lipid metabolism and gene expression in differentiated human skeletal muscle cells (myotubes) incubated for 4 days under normal (5.5 mM glucose) and hyperglycemic (20 mM glucose) conditions. Myotubes established from lean, healthy volunteers were treated with benfotiamine for 4 days. Glucose and lipid metabolism were studied with labeled precursors. Gene expression was measured using real time polymerase chain reaction (qPCR) and microarray technology.