Project description:Mice were put on either a control or low isoleucine diet and their liver transcriptomic expression was measured in both the fasting and fed state. This was to discover the impact of reducing a single branched chain amino acid on the metabolism of the liver.
Project description:Mice were put on either a control, low amino acid or low isoleucine diet at 9 weeks of age or 6 months of age and their liver transcriptomic expression was measured after 14 weeks and 18 months respectively on diet after an overnight fast. This was to discover the impact of sex, protein and isoleucine level on the metabolism of the liver.
Project description:miRNA profiling of mouse kidney cortex comparing control vs. low sodium diet + captopril treatment to induce renin expression. Two condition experiment: control vs treated; biological replicates: individual mice - 3 control, 3 treated. One replicate per array.
Project description:We investigated remodeling of the mitochondrial proteome to determine mechanisms of changes to lipid oxidation following high-fat feeding. C57BL/6J mice consumed either a high-fat diet (HFD, 60% fat) or low fat diet (LFD, 10% fat) for 12 weeks. Mice were fasted 4 hours then anaesthetized by sodium pentobarbital for tissue collection. A mitochondrial-enriched fraction was prepared from gastrocnemius muscles and underwent proteomic analysis by high-resolution mass spectrometry.
Project description:The goal of the experiment was to examine hepatic gene expression differences induced by a low protein high carbohydrate diet. We used chemically defined diets based on naturally defined diets first published and completely described in Fontana et al., 2016, Cell Reports.
Project description:Colonoscopy is the gold-standard for the evaluation of the colorectal mucosa. Colonoscopy quality indicators are the adenoma detection rate, the rate of cecal intubation and the bowel preparation.
The role of diet in preparing for colonoscopy is not fully established. Currently there is not enough evidence available to choice between 3 days of low residue diet versus 1 day.
The research hypothesis is that the low residue diet offers a non-inferior bowel preparation and an improved tolerance.
Project description:Previous studies in our laboratory have shown that low folate diet (control diet with 2mg folate/kg, low folate diet with 0.3mg folate/kg) can induce intestinal tumors in BALB/c mice. We used microarrays to compare MTHFR+/+ BALB/c mice fed control diet and MTHFR+/- BALB/c mice fed low folate diet.
Project description:Mice were put on either a control, medium or low protein diet and their liver transcriptomic expression was measured after 13 weeks on diet after an overnight fast. This was to discover the impact of sex, strain and protein level on the metabolism of the liver.