ABSTRACT: Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - JAM1.1 3a ECM III
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - JAM1.1 3a ECM I
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - JAM1.1 3a ECM II
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - GUS2 ECM III
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - GUS6 ECM III
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - MYC2.1 3 ECM III
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - MYC2.2 2 ECM III
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - MYC2.1 2 ECM III
Project description:Illumina technology was used to generate mRNA profiles of a time course of Laccaria bicolor S238N and Populus tremula x alba 717-1B4 in vitro ectomycorrhizal development. Total RNA was extracted, TruSeq mRNA Stranded libraries were constructed and and sequenced in triplicates (2 x 150 bp Illumina HiSeq3000) at the Genotoul sequencing facilities (Toulouse, France). Raw reads were trimmed for low quality (quality score 0.05), Illumina adapters and sequences shorter than 15 nucleotides and aligned to the L. bicolor v2 reference transcripts available at the JGI database https://mycocosm.jgi.doe.gov/Lacbi2/Lacbi2.home.html using CLC Genomics Workbench v8.
Project description:This study characterizes the transcriptomic alterations of Laccaria bicolor S238N during interaction with Pseudotsuga menziesii. Three time-points were analyzed, two weeks, four weeks and six weeks after inoculation and compared to the transcriptome of free-living mycelium from Laccaria bicolor S238N. We performed 9 hybridizations (NimbleGen) with samples derived from Pseudotsuga menziesii /L.bicolor mycorrhizal root tips. Samples were taken after 2,4 and 6 weeks of interaction (three biological replicates). These samples were compared to free-living mycelium from Laccaria bicolor S238N (three biological replicates). All samples were labeled with Cy3.
