ABSTRACT: Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - GUS2 ECM II
Project description:Illumina technology was used to generate mRNA profiles of a time course of Laccaria bicolor S238N and Populus tremula x alba 717-1B4 in vitro ectomycorrhizal development. Total RNA was extracted, TruSeq mRNA Stranded libraries were constructed and and sequenced in triplicates (2 x 150 bp Illumina HiSeq3000) at the Genotoul sequencing facilities (Toulouse, France). Raw reads were trimmed for low quality (quality score 0.05), Illumina adapters and sequences shorter than 15 nucleotides and aligned to the L. bicolor v2 reference transcripts available at the JGI database https://mycocosm.jgi.doe.gov/Lacbi2/Lacbi2.home.html using CLC Genomics Workbench v8.
Project description:The Poplar transcriptome was analyzed in mycorrhizal root tips in contact with Laccaria bicolor for 2 weeks. During mycorrhization the roots were treated with either 250M-BM-5m ACC, 10nM JA or 500M-BM-5M SA and compared to untreated mycorrhiza or control roots without contact to L. bicolor. In addition the poplar mutants 35S::PttACO1 and 35S::Atetr1 were used We performed 27 hybridizations (NimbleGen) with samples derived from Populus tremula x Populus alba clone 717-1B4 control roots, untreated mycorrhiza, SA-treated mycorrhiza, ACC-treated mycorrhiza and JA-treated mycorrhiza (3 biological replicates each) as well as Populus tremula x Populus tremuloides T89 control roots, mycorrhiza, 35S::PttACO1 mycorrhiza and 35S::Atetr1-1 mycorrhiza (3 biological replicates). All samples were labeled with Cy3.
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - GUS2 ECM I
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - GUS2 ECM III
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Populus tremula x alba INRA 717-1B4 roots treated with Methyl jasmonate. Samples were harvested after two weeks either from untreated control roots or from Methyl jasmonate treated roots. Paired-end (2X100bp) reads were generated and aligned to the Populus trichocarpa (http://www.phytozome.net/poplar.php) using CLC Genomics Workbench 6. mRNA profiles from Populus tremula x alba INRA 717-1B4 roots treated with Methyl jasmonate as well as from control roots were generated by paired-end (2X100bp) Illumina HiSeq sequencing. Four samples were sequenced per lane, two biological replicates per treatment.
Project description:This study characterizes the transcriptomic alterations of P. tremula x P. alba at three weeks after inoculation with the ectomycorrhizal fungus Laccaria bicolor. We performed 6 hybridizations (NimbleGen) with samples derived from Populus tremula x P. alba control roots and mycorrhizal root tips. Samples were taken after 3 weeks of interaction (three biological replicates). All samples were labeled with Cy3.
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - GUS6 ECM II
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - GUS6 ECM III
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - pRR 1 ECM
Project description:Metatranscriptome of Populus tremula X alba 717-1B4 inoculated with Laccaria bicolor S238N ECM root tips from Champenoux, France - pRR 3 ECM
