Project description:We analyze the transcriptome of Ogataea parapolymorpha wild-type and met4 mutant under sulfur limited condition to investigate the function of Met4 protein in this yeast in the absence of any sulfur source. By sequencing the mRNAs of the wild-type and mutant grown in sulfur-limited B minimal medium with or without cysteine as a sole sulfur source, we find the role of OpMet4p as a master regulator for cell homeostasis under sulfur limitation.

2019-04-19 | GSE130004 | GEO

Investigation of the contribution of Complex I to respiratory energy-coupling in glucose-grown cultures of the Crabtree-negative yeast Ogataea parapolymorpha

Project description:The methylotrophic, thermotolerant yeast Ogataea parapolymorpha (formerly Hansenula polymorpha) is an industrially relevant production host and exhibits a respiratory metabolism in the presence of oxygen. It possesses a branched respiratory chain with multiple entry points for NADH-derived electrons that differ in complexity and degree of energy conservation: proton-translocating respiratory Complex I and three putative alternative NADH dehydrogenases. To investigate the physiological importance of Complex I, wild type O. parapolymorpha and a Complex I-disrupted mutant were cultured in glucose-grown bioreactor experiments in batch, chemostat and retentostat cultivations which allowed quantitative characterization of the strains over a wide range of growth rates in the presence and absence of excess substrate.

2020-05-12 | GSE140480 | GEO

Ogataea parapolymorpha strain:CBS11895

Project description:Investigation of the contribution of Complex I to respiratory energy-coupling in glucose-grown cultures of the Crabtree-negative yeast Ogataea parapolymorpha

| PRJNA588376 | ENA

Ogataea angusta DL1 strain: Control vs. hac1 deletion mutant in exponential growth

Project description:Transcriptional profiling of hac1 deletion mutant compared to wild type (control). Wild type vs. hac1 deletion mutant of Ogataea angusta DL1 strain in exponential growth. Two biological replicates, with dye-swaps.

2015-07-22 | E-GEOD-67084 | biostudies-arrayexpress

Re-oxidation of cytosolic NADH is a major contributor to the high oxygen requirements of the thermotolerant yeast Ogataea parapolymorpha in oxygen-limited cultures

Project description:Analysis of gene expression changes in O. parapolymorpha in response to oxygen-limitation

2021-03-26 | GSE169612 | GEO

Ogataea parapolymorpha DL-1

Project description:Hansenula polymorpha telomere PCR sequencing

| PRJNA209931 | ENA