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Generation of cortical neurons through large-scale expanding neuroepithelial stem cell from human pluripotent stem cells

ABSTRACT: Generation of cortical neurons through large-scale expanding neuroepithelial stem cell from human pluripotent stem cells

PROVIDER: PRJNA714639 | ENA |

REPOSITORIES: ENA

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Generation of cortical neurons through large-scale expanding neuroepithelial stem cell from human pluripotent stem cells

Project description:We developed a serum-free, defined, and low-cost culture system for large-scale expansion of NESCs in stirred suspension bioreactors. The stable and controllable 3D system supports long-term expansion of highquality and homogeneous NESC-spheres. These NESC-spheres can be used to efficiently give rise to cortical neurons for cell therapy, disease modeling, and drug screening in future.

2021-03-15 | GSE168941 | GEO

Signal requirement for cortical potential of transplantable human neuroepithelial stem cells

Project description:The cerebral cortex develops from dorsal forebrain neuroepithelial progenitor cells. Initial expansion of the progenitor cell pool is followed by the generation of neurons of all the cortical layers and later, astrocytes and oligodendrocytes. However, the regulatory pathways that control the expansion and maintenance of the neuroepithelial progenitor cell pool are currently unknown. Here we define six basic pathway components that regulate proliferation of cortically specified human neuroepithelial stem cells (cNESCs) in vitro without the loss of developmental potential. We show that activation of FGF and inhibition of BMP and ACTIVIN A signalling are required for long-term cNESC proliferation. We also demonstrate that cNESCs preserve dorsal telencephalon-specific potential when GSK3, AKT and nuclear CATENIN-b1 activity are low. Remarkably, regulation of these six pathway components supports the clonal expansion of cNESCs. Moreover, cNESCs differentiate to lower and upper layer cortical neurons both in vitro and in vivo. Identifying the mechanisms that drive the self-renewal and fate of cNESCs decision of neuroepithelial stem cells is key to developing new stem cell-based therapeutic approaches to treat neurological conditions.

2022-02-04 | GSE184081 | GEO

Generation of cortical neurons through large-scale expanding neuroepithelial stem cell from human pluripotent stem cells

Project description: Not available

| S-EPMC7532602 | biostudies-literature

Signal requirement for cortical potential of transplantable human neuroepithelial stem cells [array]

2022-02-04 | GSE185258 | GEO

Signal requirement for cortical potential of transplantable human neuroepithelial stem cells

Project description:Signal requirement for cortical potential of transplantable human neuroepithelial stem cells

| PRJNA763125 | ENA

Signal requirement for cortical potential of transplantable human neuroepithelial stem cells [array]

Project description:Signal requirement for cortical potential of transplantable human neuroepithelial stem cells [array]

| PRJNA768415 | ENA

Transcription profiling of human embryonic stem cells and neuroepithelial cells

Project description:Comparing gene expressing profile of human embryonic stem cells and neuroepithelial cells differentiated from human embryonic stem cells.

2009-11-08 | E-MEXP-2426 | biostudies-arrayexpress

Proteomics characterisation of induced pluripotent stem cells and cortical brain organoids.

Project description:A method was developed to reproducibly produce neural retina and cortical brain regions from confluent cultures of stem cells. The spontaneously generated cortical organoids were isolated and cultured in suspension conditions for maturation. Proteomic analysis of both the original induced pluripotent stem cells and the cortical organoids demonstrated the increased presence of synaptic components, indicating maturity.

2022-05-06 | PXD025933 | Pride

Single cell transcriptome of cultured thymic cortical and medullary progenitor and stem cells and skin keratinocyte stem/progenitor cells

Project description:Purpose: The goal of this study is to characterise expanding thymic progenitor and stem cells from whole epithelial as well as cortical, medullary thymus sub-compartments. As reference, we have sequenced skin keratinocyte progenitor and stem cells Methods: Cells have been expanded for 4 passages on mouse feeder layer cells then loaded into the 10x Genomics Chromium Platform, and sequenced using Illumina HiSeq 4000. Conclusions: Our study compared thymic progenitor and stem cells from different origin and dissected in vitro properties of thymic and skin progenitor and stem cells

2023-08-30 | GSE220829 | GEO

Brain heterotopia formation by ciliopathic breakdown of neuroepithelial and blood-cerebrospinal fluid barriers

Project description:In this study, we found that ablation of genes encoding ciliary transport proteins such as intraflagellar transport homolog 88 (Ift88) and kinesin family member 3a (Kif3a) in cortical radial progenitors led to periventricular heterotopia during late mouse embryogenesis. Conditional mutation of primary cilia unexpectedly caused breakdown of both the neuroepithelial lining and the blood-choroid plexus barrier. Choroidal leakage was partially caused by enlargement of the choroid plexus in the cilia mutants. We found that the choroid plexus expressed platelet-derived growth factor A (Pdgf-A) and that Pdgf-A expression was ectopically increased in cilia-mutant embryos. Cortices obtained from embryos in utero electroporated with Pdgfa mimicked periventricular heterotopic nodules of the cilia mutant.

2023-01-10 | PXD038557 | Pride

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