Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Predicting the allergenicity of legume proteins using a PBMC gene expression assay_Pilot experiment

ABSTRACT: Predicting the allergenicity of legume proteins using a PBMC gene expression assay_Pilot experiment

PROVIDER: PRJNA714918 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Predicting the allergenicity of legume proteins using a PBMC gene expression assay_Pilot experiment

Project description:Food proteins differ in their allergenic potential. Currently, there is no predictive and validated bio-assay to evaluate the allergenicity of novel food proteins. The objective of this study was to investigate the potential of a human peripheral blood mononuclear cell (PBMC) gene expression assay to identify biomarkers to predict the allergenicity of legume proteins. PBMCs from healthy donors were exposed to weakly and strongly allergenic legume proteins. Possible biomarkers for allergenicity were investigated by exposing PBMCs to a protein pair of weakly (white bean) and strongly allergenic (soybean) 7S globulins in a pilot experiment. Gene expression was measured by RNA-sequencing and differentially expressed genes were selected as biomarkers. 153 genes were identified as having significantly different expression levels to the 7S globulin of white bean compared to soybean.

2021-03-17 | GSE168698 | GEO

Predicting the allergenicity of legume proteins using a PBMC gene expression assay

Project description:Predicting the allergenicity of legume proteins using a PBMC gene expression assay

| PRJNA714929 | ENA

Predicting the allergenicity of legume proteins using a PBMC gene expression assay_Biomarker search study

Project description:Food proteins differ in their allergenic potential. Currently, there is no predictive and validated bio-assay to evaluate the allergenicity of novel food proteins. The objective of this study was to investigate the potential of a human peripheral blood mononuclear cell (PBMC) gene expression assay to identify biomarkers to predict the allergenicity of legume proteins. PBMCs from healthy donors were exposed to weakly and strongly allergenic legume proteins. Inclusion of multiple protein pairs from 2S albumins (lupine and peanut) and 7S globulins (white bean and soybean) in a larger study, led to the selection of CCL2, CCL7, and RASD2 as biomarkers to distinguish weakly from strongly allergenic proteins.

2021-03-17 | GSE169030 | GEO

Predicting the allergenicity of legume proteins using a PBMC gene expression assay_Biomarker search study

Project description:Predicting the allergenicity of legume proteins using a PBMC gene expression assay_Biomarker search study

| PRJNA714933 | ENA

Predicting the allergenicity of legume proteins using a PBMC gene expression assay.

Project description: Not available

| S-EPMC8042678 | biostudies-literature

Predicting the allergenicity of legume proteins using a PBMC gene expression assay

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-03-17 | GSE169031 | GEO

Altered porcine PBMC proteome and secretome after stimulation with BanLec

Project description:With this experiment, we aimed at showing changes in the proteome and secretome of porcine peripheral blood mononuclear cells (PBMC) after stimulation with Banana Lectin (BanLec).

2021-08-26 | PXD027505 | Pride

Expression data from iPSCs, iPSC-derived neurons, PBMC-derived Monocytes, PBMC-derived Macrophages, PBMC-derived induced microglia

Project description:iPSCs, iPSC-derived neurons, PBMC-derived Monocytes, PBMC-derived Macrophages, PBMC-derived induced microglia

2018-12-05 | GSE123349 | GEO

Legume Diet Satiety Pilot Study

Project description:This pilot study is designed to test the effects of a high legume (dried bean) diet on hunger and other indicators of health over the course of eight weeks, compared to a more conventional healthy diet.

| 2176646 | ecrin-mdr-crc

Transcription profiling of Medicago trunculata leaf tissue over-expressing Legume Anthocyanin Production

Project description:Transcription profiling of leaf tissue from Medicago truncatula over-expressing Legume Anthocyanin Production.

2009-10-13 | E-MEXP-1854 | biostudies-arrayexpress