Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Exosomal-miRNA sequencing of optineurin gene knockdown BV2 cells and wild type BV2 cells

ABSTRACT: Exosomal-miRNA sequencing of optineurin gene knockdown BV2 cells and wild type BV2 cells

PROVIDER: PRJNA715477 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Exosomal-miRNA sequencing of optineurin gene knockdown BV2 cells and wild type BV2 cells

Project description:To compare the miRNA profiles in exosomes derived from optineurin gene knockdown (Optn-KD)BV2s cells and wild type (WT) BV2 cells, BV2 cells were divided into two groups, Optn-KD group or WT group. We treated the Optn-KD group with Optn siRNA for 24 hours. Then, MiRNA profiles for each group were examined by Illumina HiSeq4000. We found differentiated miRNA profiles in the two groups. Compared with WT BV2 cell-derived exosomes, 4 miRNAs (miR-125b-5p, miR-351-5p, miR-505-5p, miR-novel-chr8_37700) were up-regulated and 2 miRNAs (miR-574-5p, miR-99b-5p) were down-regulated in Optn-KD BV2 cell-derived exosomes. This study provides a framework for characterizing the exosomal-miRNAs in Optn-KD BV2 cells.

2021-03-19 | GSE169175 | GEO

Microglial BV2-derived exosomal microRNA sequencing in heatstroke

Project description:We performed exosomal microRNA sequencing on BV2 in heatstroke, to shed light on the expression and function changes of microglia in heatstroke. We then performed gene expression profiling analysis using data obtained from microRNA-seq of 2 groups.

2022-10-08 | GSE210897 | GEO

RNA sequencing in wild type and GCH1-knockdown (KD) BV2 microglial cells

Project description:We conducted high-throughput sequencing and bioinformatics analysis of GCH1-KD BV2 microglial cells treated with adenovirus. Their RNA was extracted and analyzed, and the results were verified by quantitative real-time polymerase chain reaction (PCR). This study explored the lncRNAs, miRNAs, circRNAs and mRNAs regulated by GCH1 and revealed a possible mechanism of GCH1 in microglia activation.

2023-01-31 | GSE164836 | GEO

Small RNA sequencing in wild type and GCH1-knockdown (KD) BV2 microglial cells

Project description:We conducted small RNA sequencing and bioinformatics analysis of GCH1-KD BV2 microglial cells treated with adenovirus. Their RNA was extracted and analyzed, and the results were verified by quantitative real-time polymerase chain reaction (qRT-PCR). This study explored the miRNAs and mRNAs regulated by GCH1 and revealed a possible mechanism of GCH1 in microglial activation.

2023-01-05 | GSE165204 | GEO

Chip-seq data of BV2 cells by anti-Nr4a2 antibody

Project description:Glioma is the most common type of intracranial tumor and accounts for 75% of malignant brain tumors. Glioblastoma (GBM) is the most malignant form of glioma, with strong invasive capabilities and a high relapse rate even after treatment. Here, our RNA seqencing analysis of BV2 cells treated by H2O2 and si-Nr4a2 reveal the effect of oxdiative stress and Nr4a2-knockdown on phenotype of microglia. Chip-seqencing andlysis of BV2 cells by anti-Nr4a2 antibody is to find the target genes regulated by Nr4a2. Overall, our results reveal that Nr4a2 as the major transcription factors for microglia involved in immunosuppression of glioma.

2023-01-08 | GSE185759 | GEO

Exosomal miRNA signatures of RAW 264.7 cells

Project description:To investigate the exosomal miRNA changes under LPS treatment in RAW 264.7 cells, 2 μg/mL LPS were added into complete medium to incubate RAW 264.7 cells. And then The exosomes were isolated and tested the exosomal miRNAs change using microarray.

2023-04-30 | GSE149630 | GEO

JEV Infected BV2 Cells with Proteomics

Project description:We sought to paint a panoramic picture of JEV-infected BV2 cells. High depth TMT labelling for quantitative proteomics was used to assess JEV infection with BV2 and compared immune response profiles at 6h, 12h, and 24h post-infection (hpi).According to gene ontology and KEGG enrichment analysis, immune response-related proteins were enriched among the elevated proteins.

2022-09-09 | PXD033575 | Pride

LPS and Telmisartan co-treatment of microglial BV2 cells

Project description:LPS and Telmisartan co-treatment of microglial BV2 cells.

2018-07-02 | GSE108669 | GEO

Mus musculus strain:BV2 cells

Project description:Mus musculus strain:BV2 cells Transcriptome or Gene expression

| PRJNA258332 | ENA

LPS, Telmisartan and GW9662 co-treatment of microglial BV2 cells

Project description:LPS, Telmisartan and GW9662 co-treatment of microglial BV2 cells.

2018-07-02 | GSE108670 | GEO