Project description:Although periodontitis is a widespread disease, its molecular mechanisms in human oral cells are not fully understood. Therefore, we established cell lines from the human oral cavity, including gingival keratinocytes (GK), osteoblastic lineage cells from the alveolar bone (OLAB), PDL fibroblasts (PDLF) and cementum cells (CC). Using label-free quantitative mass spectrometry, we investigated changes of the proteome of healthy human oral cells after co-cultivation with Aggregatibacter actinomycetemcomitans and Eikenella corrodens for 24 h, for the first time. Our findings show specific protein profiles for each of the human oral cell lines. This will help to understand pathologic mechanisms in periodontitis and related diseases, such as rheumatoid arthritis, diabetes mellitus or atherosclerotic vascular diseases.
