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Transcriptional profiling of basilar artery and basilar terminus intima and media in rabbits after bilateral carotid ligation

ABSTRACT: Transcriptional profiling of basilar artery and basilar terminus intima and media in rabbits after bilateral carotid ligation

PROVIDER: PRJNA716071 | ENA |

REPOSITORIES: ENA

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Transcriptional profiling of basilar artery and basilar terminus intima and media in rabbits after bilateral carotid ligation

Project description:New Zealand white rabbits underwent bilateral carotid ligation to increase flow in the basilar artery (BA) or sham surgery. 24 hours post-surgery, the BA and basilar terminus (BT) were embedded for sectioning. The intima and media were separately laser-microdissected from the sections, and whole transcriptome RNA-seq was performed on each tissue in order to more thoroughly characterize potential molecular signals between endothelium and smooth muscle during flow-induced arterial remodeling.

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Microarray-based gene expression of rabbit basilar artery smooth muscle cells exposed to hemodynamic insult

Project description:High-flow causes the remodeling of arteries, in which smooth muscle cells play an important role. To know the profile of smooth muscle gene expression under high-flow conditions in vivo, flow of rabbit basilar artery was increased by ligation of both common carotid arteries. Microarrays were performed to profile the gene expression of smooth muscle cells isolated from rabbit basilar artery. Expression profiles indicate 43603 differentially expressed genes in smooth muscle cells exposed to high-flow insult compared with the sham control, of which 1470 genes were upregulated and 780 genes downregulated using 2 fold-changes and P<0.05 as a cut-off. Bilateral common carotid arteries of female New Zealand White rabbits were ligated to increase vascular flow.The control group was performed the same procedure to expose the CCAs without ligation. Rabbits were euthanized at day 5 after ligation or exposure of bilateral CCAs in both groups (n=3 for each group). The rabbits used and all procedures in this study were approved by the local Institutional Animal Care and Use Committee. Smooth muscle cells were isolated. After euthanization of rabbits, the whole basilar arteries were removed. The arteries were cleaned in PBS buffer,cannulated and perfused at a constant flow with a cocktail which contains PBS and 0.4 mg/ml elastase (Sigma) and 1 mg/ml collagenase (type 1A, Sigma). After an incubation time of 45 min, the tissue left was removed and stored in PBS. SMCs were released from the artery by trituration. Then Total RNA was extracted and gene chip tests were performed.

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Gene expression changes accompanying vascular tortuosity

Project description:To gain insight into vascular tortuosity, we analyzed gene expression in normal and tortuous vessels. We induced vascular tortuosity in basilar artery using bilateral common carotid artery ligation in One-year old SD rats. Four weeks after the ligation, total RNA was extracted. Equal amounts of RNA from four rats were pooled and 50 ng RNA was subjected to microarray analysis. Pooled RNA from four Sham operated rats were used as a control.

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Project description:This analysis compares gene expression in human carotid plaques with gene expression in major tissues and cell types in the human body (GSE1133, Su et al. 2004). Experiment Overall Design: Human carotid endarterectomies were isolated from six subjects with symptomatic carotid artery disease.

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Glucagon-like peptide-1 suppresses sympathetic activation via peripheral chemosensory inhibition

Project description:In order to investigate the molecular basis of carotid body chemoreceptor sensitisation in the Spontaneously Hypertensive Rat (SHR) we have sequenced the transcriptomes of bilateral carotid body samples from aged-matched, male SHR and control Wistar-Kyoto rats.

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