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Comprehensive analysis of pathological changes in hip joint capsule of patients with developmental dysplasia of the hip

ABSTRACT: Comprehensive analysis of pathological changes in hip joint capsule of patients with developmental dysplasia of the hip

PROVIDER: PRJNA716732 | ENA |

REPOSITORIES: ENA

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Comprehensive analysis of pathological changes in hip joint capsule of patients with developmental dysplasia of the hip

Project description:Aims: Developmental dysplasia of the hip (DDH) is a complex musculoskeletal disease that occurs mostly in children. This study aimed to investigate the molecular changes in the hip joint capsule of patients with DDH. Results: More than one thousand genes were differentially expressed in hip joint capsules between healthy controls and DDH. Both gene ontology (GO) and Kyoto encyclopedia of genes and genome (KEGG) analyses revealed that extracellular matrix (ECM) modifications, muscle system processes, and cell proliferation were markedly influenced by the differentially expressed genes. Conclusion: DDH is associated with the loss of collagen fibers and fibroblasts, which may cause loose joint capsule formation. However, the degree of differentiation of fibroblasts to myofibroblasts needs further studies.

2022-03-21 | GSE169494 | GEO

Periostin induces early articular cartilage degeneration in developmental dysplasia of the hip

Project description:Developmental dysplasia of the hip (DDH) is one of the significant risk factors for hip osteoarthritis. In order to investigate the factors that induce early articular cartilage degeneration of the hip joints that are exposed to reduced dynamic loads arising from hip dislocation , we created rodent models of hip dislocation by swaddling. Notably, expression of periostin (Postn) was increased in the acetabular articular cartilage of the DDH models; Postn was a candidate gene associated with early articular cartilage degeneration. We showed that early articular cartilage degeneration was suppressed in Postn-/- DDH mice. Furthermore, a microgravity environment induced the expression of Postn in chondrocytes through STAT3 signaling. Postn induced catabolic factors, interleukin-6 and matrix metalloproteinase 3, in articular chondrocytes through integrin-nuclear factor κB signaling. Additionally, interleukin-6 stimulated Postn expression through STAT3 signaling. Thus, Postn plays a critical role in early articular cartilage degeneration associated with hip dislocation.

2022-07-20 | GSE173637 | GEO

Differential gene expression in the posterior joint capsule for immobility in mice

Project description:To explore the factors involved in arthrofibrosis, we performed a genome-wide screening for mRNA expressed in the posterior capsule after immobilization in mice. A gene ontology term analysis for differentially expressed genes after 2 weeks immobilization indicated that the genes involved in the extracellular region were particularly enriched. A heatmap demonstrated that joint immobilization significantly altered the expressions of genes related to the extracellular region. The MA plot indicated that, compared to naïve mice, 1654 genes were upregulated more than 2-fold, and 985 genes were downregulated more than 2-fold in immobilized mice. The MA plot and heatmap visually revealed dynamic changes in the expression of genes in immobilized posterior capsules compared to those in naïve posterior capsules. Our study represents the first detailed analysis of mouse joint capsule transcriptomes, with biologic replicates, generated by RNA-seq technology. RNA-seq based transcriptome characterization would expedite genetic network analyses and clarify Unresolved pathophysiology of joint contractures.

2024-01-01 | GSE223970 | GEO

Developmental Dysplasia of the Hip DDH

Project description:The National Joint Registry for England and Wales (NJR) has become established as one of the largest repositories of clinical information about patients undergoing hip and knee replacement. The primary aim of this proposal is to examine the feasibility of adding value to that clinical information by partnering it with a DNA dataset to allow its use to be extended to the study of the underlying pathogenesis of diseases of the hip and knee, and the complications of their treatment. We will do this 1) by establishing a mechanism for accessing and linking pseudo-anonymised demographic and phenotype data between the NJR database and the proposed DNA Biobank; 2) by validating the collection of postal DNA samples from consenting NJR patients; and 3) by genotyping genome-wide 900 patients with the condition developmental dysplasia of the hip (DDH) to find variants that play a role in this heritable condition. The successful demonstration that the NJR can be used to establish a DNA Biobank will provide a powerful platform to examine the genetic basis for a wide range of joint diseases, and the complications of their treatment. This proposal is to carry out the genotyping portion of this project on the current CoreExome array

| EGAS00001000916 | EGA

Gene Expression in Hip Soft Tissues in a Natural Canine Model of Developmental Dysplasia of the Hip

Project description:We provide data that compares global gene expression in dysplastic hip compared to control hip tissue in dogs.

2018-11-27 | GSE112874 | GEO

Gene Expression in Hip Soft Tissues in a Natural Canine Model of Developmental Dysplasia of the Hip

Project description:Gene Expression in Hip Soft Tissues in a Natural Canine Model of Developmental Dysplasia of the Hip

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Hip Osteoarthritis Functional Genomics

Project description:The aim of this work is to apply an integrated systems approach to understand the biological underpinnings of hip osteoarthritis that culminates in the need for total joint replacement (TJR). This study is a feasibility pilot that integrates functional genomics data from diseased and non-diseased tissues of OA patients who have undergone TJR. For each tissue, we characterised epigenetic marks (methylation), gene transcription (RNASeq) and expression (quantitative proteomics). We also generated genotype data on the HumanCoreExome array for each individual. This data is part of a pre-publication release.

2018-05-14 | PXD006673 | Pride

miRNA and mRNA transcriptome profiling of chondrocytes in proliferative zone of acetabular cartilage in developmental dysplasia of the hip

Project description:Developmental dysplasia of the hip (DDH) is a complex and multifactorial disease with an unclear pathogenesis, and the non-coding RNAs have been found important effect in many diseases. However, their contributions to the pathogenesis of DDH are not well-established. Growth of the acetabular cartilage mainly depends on the regulation of chondrocytes in proliferative zone. Here, we report on some new regulatory mechanisms of gene expression underlying the development of DDH by transcriptome profiling of proliferative zone microdissected from the acetabular cartilage. These results provide original insight into the roles of non-coding RNAs in DDH and provide a valuable resource for further analyses of molecular mechanisms and signaling networks.

2024-04-03 | GSE240299 | GEO

Genome-wide DNA methylation study of hip and knee osteoarthritic and healthy cartilage reveals embryonic organ and skeletal system morphogenesis as major pathways involved in osteoarthritis

Project description:Cartilage samples were collected from hip or knee joint replacement patients either due to primary OA or hip fractures as controls. DNA was extracted from the collected cartilage and assayed by Illumina Infinium HumanMethylation450 âBeadChip array, which allows for the analysis of >480,000 CpG sites. Bisulphite converted DNA from 5 hip osteoarthritic, 6 knee osteoarthritic and 7 hip healhty cartilage samples were hybridised to the Illumina Infinium HumanMethylation450 âBeadChip array

2016-07-01 | E-GEOD-73626 | biostudies-arrayexpress

Genomic variants associating with canine hip dysplasia

Project description:Validation study of SNPs associating with canine hip dysplasia

2020-11-05 | PRJEB41117 | EVA