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BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae (ChEC-Seq)

ABSTRACT: BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae (ChEC-Seq)

PROVIDER: PRJNA718270 | ENA |

REPOSITORIES: ENA

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BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae (ChEC-Seq)

Project description:Human BET family members are promising targets in the therapy of cancer and immunoinflammatory diseases, but their mechanism of action and functional redundancies are poorly understood. Yeast BET factors Bdf1/2 were previously proposed to act as anchors for coactivator TFIID. We investigated their genome wide roles in transcription and found that, while they cooperate with TFIID at many genes, their contributions to transcription are often significantly different. Bdf1/2 co-occupy the majority of yeast promoters and affect preinitiation complex formation by participating in recruitment of TFIID, Mediator and basal factors to chromatin. Surprisingly, we discovered that hypersensitivity of genes to Bdf1/2 depletion results from combined defects in initiation of transcription and early elongation. Bdf1/2 are critical components of yeast transcriptional machinery with many functional similarities to human BET proteins, most notably Brd4.

2021-06-09 | GSE171062 | GEO

BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae (RNA-Seq)

2021-06-09 | GSE171065 | GEO

BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae (ChIP-Seq)

2021-06-09 | GSE171063 | GEO

BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae

Project description:BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae

| PRJNA718271 | ENA

BET family members Bdf1/2 modulate global transcription initiation and elongation in <i>Saccharomyces cerevisiae</i>.

Project description: Not available

| S-EPMC8266393 | biostudies-literature

BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae (ChIP-Seq)

Project description:BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae (ChIP-Seq)

| PRJNA718273 | ENA

BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae (RNA-Seq)

Project description:BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae (RNA-Seq)

| PRJNA718274 | ENA

BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-06-09 | GSE171067 | GEO

Yeast Bdf1 interactome - Bdf1 Bromodomains are Essential for Meiosis and the Expression of Meiotic-Specific Genes

Project description:Bromodomain and Extra-terminal motif (BET) proteins play a central role in transcription regulation and chromatin signalling pathways. In yeast, Bdf1 and its homologous protein Bdf2 are partially redundant and the deletion of both genes is lethal. Most of Bdf1 functions have been investigated in vegetative cells even if this protein is essential for sporulation. In this study, we explored for the first time the functional role of the Bdf1 bromodomains during the sporulation program. Extensive purification of Bdf1 partners identified two independent complexes with Bdf2 or the Swr1 complex. However, none of them are required for meiotic gene regulation or to complete sporulation. Taken together, our results unveil a new role for Bdf1 as a meiotic-specific gene transcriptional regulator independently of its predominant protein partners.

2017-01-11 | PXD005227 | Pride

High resolution genome-wide occupancy in Candida species using ChEC-seq

Project description:Chromatin endogenous cleavage (ChEC) uses fusion of a protein of interest to micrococcal nuclease (MNase) to target calcium-dependent cleavage to specific genomic loci in vivo. Here we report the combination of ChEC with high-throughput sequencing (ChEC-seq) to determine genomic occupancy of the generalist transcription factor Nsi1 and the catalytic subunit of the SWI/SNF chromatin remodelling complex Snf2 in the opportunistic yeast Candida albicans. Time-based analysis of ChEC-seq data reveals two classes of sites for each transcriptional regulator, one exhibiting rapid cleavage during the first 5 min with robust consensus motifs and the second showing slow cleavage at largely unique sites with low-scoring motifs. The ChEC-seq procedure described here will allow a high-resolution genomic location definition which will enable a better understanding of transcriptional regulatory circuits that govern fungal fitness and drug resistance in these medically important fungi.

2020-05-08 | GSE150063 | GEO

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