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Bioengineered embryoids mimic post-implantation development in vitro [EmbryoidsTruseq3]

ABSTRACT: Bioengineered embryoids mimic post-implantation development in vitro [EmbryoidsTruseq3]

PROVIDER: PRJNA718750 | ENA |

REPOSITORIES: ENA

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Bioengineered embryoids mimic post-implantation development in vitro [Embryoids10XG]

Project description:Bioengineered embryoids mimic post-implantation development in vitro [Embryoids10XG]

| PRJNA718748 | ENA

Bioengineered embryoids mimic post-implantation development in vitro [Embryoids10XG]

Project description:Here, we report a bioengineering-inspired approach to simultaneously coax mouse embryonic stem cells (ESCs) into hundreds of uniform epiblast-like (EPI) aggregates in a solid matrix-free manner. When co-cultured with mouse trophoblast stem cell (TSC) aggregates, the resulting hybrid structures initiate gastrulation-like events and undergo axial morphogenesis to yield structures, termed EpiTS embryoids, with a pronounced anterior development, including brain-like regions.

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Bioengineered embryoids mimic post-implantation development in vitro [EmbryoidsTruseq3]

2021-04-12 | GSE171211 | GEO

Bioengineered embryoids mimic post-implantation development in vitro.

Project description: Not available

| S-EPMC8390504 | biostudies-literature

Single-cell RNA sequencing data of stem cell-derived microfluidic human embryoids, NODAL-KO human embryoids and chimpanzee embryoids

Project description:Transcriptome at the single-cell level of a human embryoid model, which recapitulates aspects of lineage diversification and three-dimensional tissue architecture of the human embryo from the implantation to the onset of gastrulation, was profiled at different time points. Similarly, chimpanzee embryoids were generated and profiled to reveal transcriptome dynamics during the early post-implantation chimpanzee development. Our comparative transcriptome analyses reveal a critical role of NODAL signaling in human mesoderm specification, which is functionally validated by generating embryoids with NODAL-knockout (KO) hPSCs.

2021-10-13 | GSE185643 | GEO

Spatial transcriptomic profiling of Patient and Patient-corrected bioengineered vascular grafts.

Project description:Spatial transcriptomic profiling of two replicates of Patient and two replicates of Patient-corrected bioengineered vascular grafts harvested at week 8 post-implantation.

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Murine uterus expression profile during Peri-implantation: Pre-implantation vs. Post-implantation

Project description:Anaylsis differentially expressed genes of mouse peri-implanted uteri comparing pre-implantation uteri (Day2, Day3 and Day4) with post-implantation uteri (Day6, Day7 and Day8) by microassay. This study has built a meaningful basis for future investigation in elucidating the molecular nature of maternal-fetal interactions during pregnancy establishment and maintenance. Pre-implantation uteri VS. Post-implantation uteri. Three biological replicates of each experiments: pre-implantation uteri (Day2, Day3 and Day4): 234, 234①, 234②; 3 mixture of post-implantation uteri (Day6, Day7 and Day8): 678, 678①, 678②. Two hybridizations were performed by using a reverse fluorescence strategy (Cy3, Cy5) for each sample.

2011-06-30 | E-GEOD-26615 | biostudies-arrayexpress

Single-cell RNA sequencing data of stem cell-derived microfluidic human embryoids, NODAL-KO human embryoids and chimpanzee embryoids

Project description:Single-cell RNA sequencing data of stem cell-derived microfluidic human embryoids, NODAL-KO human embryoids and chimpanzee embryoids

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Single-cell RNA-sequencing of mouse embryoids at Day 8

Project description:Generating properly organized and differentiated embryonic structures in vitro from aggregates of pluripotent cells remains a major challenge to overcome. In the living embryo, the interplay between morphogens and their antagonists set up gradients of activity that instruct cells about their fate, leading to patterning and morphogenesis. Here we show that experimentally engineering a morphogen signaling center within an aggregate of mouse pluripotent stem cells is sufficient to mimic in vitro the function of an embryo organizer and to trigger embryonic development. The resulting embryoids are extensively patterned along their anterior-posterior and dorsal-ventral axes, with formation of three germ layers through a process of gastrulation and differentiation of germ layer derivatives similar to those of a neurula-stage mouse embryo.

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Efficient generation of ETX embryoids that recapitulate the entire window of murine egg cylinder development

Project description:The murine embryonic–trophoblast–extra-embryonic endoderm (ETX) model is an integrated stem cell–based model to study early postimplantation development. It is based on the self-assembly potential of embryonic, trophoblast, and hypoblast/primitive/visceral endoderm-type stem cell lines (ESC, TSC, and XEN, respectively) to arrange into postimplantation egg cylinder–like embryoids. Here, we provide an optimized method for reliable and efficient generation of ETX embryoids that develop into late gastrulation in static culture conditions. It is based on transgenic Gata6-overproducing ESCs and modified assembly and culture conditions. Using this method, up to 43% of assembled ETX embryoids exhibited a correct spatial distribution of the three stem cell derivatives at day 4 of culture. Of those, 40% progressed into ETX embryoids that both transcriptionally and morphologically faithfully mimicked in vivo postimplantation mouse development between E5.5 and E7.5. The ETX model system offers the opportunity to study the murine postimplantation egg cylinder stages and could serve as a source of various cell lineage precursors.

2023-01-25 | GSE206132 | GEO

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