Project description:The chromatin landscape of developing erythroblasts changes dramatically during differentiation. We have profiled the change in Gata2 binding in primary erythroid cells entering terminal differentiation using CUT&RUN.
Project description:The chromatin landscape of developing erythroblasts changes dramatically during differentiation. We have profiled the change in Gata1 binding in primary erythroid cells entering terminal differentiation using CUT&RUN.
Project description:The chromatin landscape of developing erythroblasts changes dramatically during differentiation. We have profiled the change in Nf-e2 binding in primary erythroid cells entering terminal differentiation using CUT&RUN.
Project description:The chromatin landscape of developing erythroblasts changes dramatically during differentiation. We have profiled the localisation of H3K4me3 in primary erythroid cells entering terminal differentiation using CUT&RUN.
Project description:The chromatin landscape of developing erythroblasts changes dramatically during differentiation. We have profiled the localisation of H3K4me1 in primary erythroid cells entering terminal differentiation using CUT&RUN.
Project description:The chromatin landscape of developing erythroblasts changes dramatically during differentiation. We have profiled the localisation of H3K27me3 in primary erythroid cells entering terminal differentiation using CUT&RUN.
Project description:SOX6 CUT&RUN on HUDEP1 over expressing SOX6-Flag. The experiment is done using and anti Flag Ab to assist the genome wide binding profile of SOX6 in HUDEP1 (Human Umbilical cord blood-Derived Erythroid Progenitor-1).
Project description:GATA transcription factors, particularly GATA2 and GATA3 are selectively expressed in the extraembryonic trophoblast lineage and regulates gene expression to promote trophoblast self-renewal during mammalian development. However, we have a poor understanding about the contribution of these GATA factors, in the process of syncytiotrophoblast (SynT) development. Thus, the goal of this study is to define the importance of GATA2 and GATA3 in orchestrating a global gene expression program that poises and commits mammalian trophoblast stem cells to the SynT lineage. Using the cut and run technique for GATA2 and GATA3 antibodies in the human trophoblast stem cells we are aiming to identify the direct targets of the GATA transcription factors that are essential for determining the SynT lineage. Three individual experiments were performed.