Project description:Terminally maturing erythroblasts have dynamic changes in transcription-related chromatin modifications and RNA Polymerase II occupancy
Project description:Terminally maturing erythroblasts have dynamic changes in transcription-related chromatin modifications and RNA Polymerase II occupancy (RNA-Seq)
Project description:Terminally maturing erythroblasts have dynamic changes in transcription-related chromatin modifications and RNA Polymerase II occupancy (ChIP-Seq 2)
Project description:Terminally maturing erythroblasts have dynamic changes in transcription-related chromatin modifications and RNA Polymerase II occupancy (ChIP-Seq 1)
Project description:Transcriptome analysis of total RNA samples from FACS sorted erythroblasts To further verify the role of cytokine triggered inflammation signaling in erythropoiesis, we decided to follow the profile of gene expression in erythroblasts using DNA microarrays. To elucidated the gene expression pattern in cytokine, especially IL-6, challenged erythroblasts, in vitro IL-6 treated erythroblasts in EPO containing medium both at day 1 and day 2 were collected. We also sorted the erythroblasts at different stages (Proerythroblasts(I),Basophilic(II) and polychromatic(III) erythroblasts,Orthochromatic(IV) erythroblasts) from both DWT and DKO bone marrow cells through fluorescence activated cell sorting. Total RNA were extracted and processed to hybridize with Affymetrix mouse Clariom S microarrays.
Project description:We report that Setd8 is essential for murine erythropoeisis. Setd8 null erythroblasts have severe defects in cell cycle progression, chromatin condensation,and heterochromatin integrity. They also have dysregulated gene expression.
Project description:Erythroblasts cultured from mobilized CD34+ cells from six healthy adult human donors were used to generate an erythroblast transcriptome. Cellular maturation was maintained including enucleation. On culture day 14 total RNA was isolated (see PMID: 23798711 for details). These RNA-Seq profiles were generated after flow cytometric sorting (live cell gating of culture Day 14 erythroblasts according to forward and side scatter).
Project description:Erythroblasts cultured from six healthy commercial available cord blood CD34+ cells were used to generate an cord blood erythroblast transcriptome. Cellular maturation was maintained including enucleation. On culture day 14 total RNA was isolated (see PMID: 23798711 for details). These RNA-Seq profiles were generated after flow cytometric sorting (live cell gating of culture Day 14 erythroblasts according to forward and side scatter).