Project description:Development of Pectoralis major has been investigated through gene expression analysis in comparing animals receiving a restricted diet in P and Ca (NC), a normal diet with sufficient level of P and Ca (PC), and a restricted diet supplemented with phytase (Phy1000). We used microarrays (ChiGene-1_0-st) to evaluate gene expression underlying pathways affectd by the diet and/or the phytase supplementation and to identify classes of differentially expressed genes.
Project description:The experiment aimed at investigating the profiles of two histone marks (H3K27me3 and H3K4me3) in muscle tissues (Pectoralis major) of 35 days-old control (n=3) and thermally-manipulated (cyclic rise in egg incubation temperature of 1.5°C from days 7 to 16 of embryogenesis; n=3) chickens.
Project description:miRNA expression was analysed of samples derived from M. pectoralis of day 10 and day 13 chicken embryos and day 35 broiler after variation of incubation temperature