Project description:In the current project we have carried out the first proteogenomic analysis of a parasitic helminth. This has allowed us to significantly improve the genome annotation and provide a robust characterisation of the ES proteome from the adult N. americanus. Our findings show important information on key families of proteins with both known and unknown functions which may be instrumental for host-parasite interactions, parasite survival and immune regulation. Description of these proteins is useful for informing future identification of vaccine and drug targets, diagnostics and novel therapeutics for treating autoimmune and allergic diseases.
Project description:Transcriptional profiling of larval stage I - IV of Homarus americanus, for assessment of stage specific developmental gene expression. Individuals from each stage were gathered in July of 2009 and 2010. A reference design was used to permit comparison of all stages. Study aims included assessment of global gene expression of larval development of healthy larvae, and identifying novel molecular pathways involved in H. americanus development.
Project description:These data belong to a metabolic engineering project that introduces the reductive glycine pathway for formate assimilation in Cupriavidus necator. As part of this project we performed short-term evolution of the bacterium Cupriavidus necator H16 to grow on glycine as sole carbon and energy source. Some mutations in a putiative glycine transporting systems facilitated growth, and we performed transcriptomics on the evolved strain growing on glycine. Analysis of these transcriptomic data lead us to the discovery of a glycine oxidase (DadA6), which we experimentally demonstrated to play a key role in the glycine assimilation pathay in C. necator.
