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Human neurovascular unit chip with a functional blood-brain barrier models brain-infection mechanism of a fungal meningitis pathogen

ABSTRACT: Human neurovascular unit chip with a functional blood-brain barrier models brain-infection mechanism of a fungal meningitis pathogen

PROVIDER: PRJNA721424 | ENA |

REPOSITORIES: ENA

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Human neurovascular unit chip with a functional blood-brain barrier models brain-infection mechanism of a fungal meningitis pathogen

Project description:Reconstitution of a neurovascular unit model with blood-brain barrier (BBB) function is of great importance for drug development targeting cerebral diseases and study of brain disease mechanisms. In this study, we describe a human neurovascular unit chip designed by reconstituting necessary cellular and extracellular components in microfluidic devices. Dynamic three-dimensional co-culture of human cells (neural stem cells, brain microvascular endothelial cells, and brain vascular pericytes) in microfluidics with a stepwise unidirectional flow successfully established the chip with BBB-mimetic structural and functional features to be an effective barrier for drugs and cytokines. Furthermore, we showed the utility of the chip by modeling the neurotropic behaviors and BBB penetration process of a global fungal meningitis pathogen, Cryptococcus neoformans. This chip is the first in vitro experimental model for monitoring neurotropism of C. neoformans and would contribute to the investigation of various cerebral disorders, including microbial infectious diseases and their corresponding drug development.

2021-04-13 | GSE171937 | GEO

Type 2 Diabetes Mellitus is Associated with Transcriptome Alterations in Cortical Neurones and Associated Neurovascular Unit Cells in the Ageing Brain

Project description:Type 2 diabetes mellitus (T2D), characterised by peripheral insulin resistance, is a risk factor for dementia. In addition to its contribution to small and large vessel disease, T2D may directly damage cells of the brain neurovascular unit. In this study, we investigated the transcriptomic changes in cortical neurones, and associated astrocytes and endothelial cells of the neurovascular unit, in the ageing brain

2021-01-11 | GSE161355 | GEO

The endfeetome, a astroglial translatome of the neurovascular unit

Project description:We aimed to identify astroglial transcripts preferentially translated in the neurovascular unit. The translatome of whole astrocytes extracted following the bacTRAP protocol was compared to a translatome of astrocyte perivascular endfeet.

2017-01-27 | GSE72937 | GEO

Project description:The neurovascular unit, which includes neurons, glial cells, and vascular cells, plays crucial roles in the onset and progression of Alzheimer’s disease (AD). Therefore, effective drugs against AD should be able to target the multi-cellular neurovascular unit and the therapeutic relationships among neurovascular cells should be defined. We aimed to examine the therapeutic effects of an herbal remedy with multi-targeting capabilities, ukgansan (UGS), using in vitro models of AD, and to assess the similarity among neurovascular cells in terms of a therapeutic network.

2019-07-18 | GSE134414 | GEO

mRNA and miRNA expression changes at the neurovascular unit reveal a role for autophagy and DNA binding in the cerebral microvasculature of the ageing brain (human)

Project description:Objective: To examine changes in the transcriptome of the neurovascular unit in ageing individuals

2019-07-29 | GSE127710 | GEO

mRNA and miRNA expression changes at the neurovascular unit reveal a role for autophagy and DNA binding in the cerebral microvasculature of the ageing brain (mouse)

Project description:Objective: To examine changes in the transcriptome of the neurovascular unit in ageing individual mice

2019-07-29 | GSE127709 | GEO

Long-Term Gene Expression Dynamics at the Neurovascular Unit After Cerebral Ischemia/Reperfusion Injury in Mice

Project description:With increasing distribution of endovascular stroke therapies, transient middle cerebral artery occlusion in mice depicts a relevant patient population with recanalized M1 occlusion. This study provides a well-controlled transcriptome dataset of short and long-term transcriptomic changes at the neurovascular unit in cerebral ischemia/reperfusion injury.

2020-03-10 | GSE131193 | GEO

Endothelial-neuronal coupling revealed in a decoupled neurovascular unit based Organ-on-Chip approach.

Project description:This data set is for the article: Endothelial-neuronal coupling revealed in a decoupled neurovascular unit based Organ-on-Chip approach.

2017-08-24 | MSV000081480 | MassIVE

The neurovascular unit is affected in GRN-associated frontotemporal dementia

Project description:The neurovascular unit is affected in GRN-associated frontotemporal dementia

| PRJNA684945 | ENA

Rescue of Impaired Blood-Brain Barrier in a Tuberous Sclerosis Complex Patient Derived Neurovascular Unit

Project description:Tuberous sclerosis complex (TSC) is a multi-system genetic disease that causes benign tumors in the brain and other vital organs. The most debilitating symptoms result from involvement of the central nervous system and lead to a multitude of severe symptoms including seizures, intellectual disability, autism, and behavioral problems. TSC is caused by heterozygous mutations of either theTSC1orTSC2gene. Dysregulation of mTOR kinase with its multifaceted downstream signaling alterations is central to disease pathogenesis. Although the neurological sequelae of the disease are well established, little is known about how these mutations might affect cellular components and the function of the blood-brain barrier (BBB). We generated disease-specific cell models of the BBB by leveraging human induced pluripotent stem cell and microfluidic cell culture technologies. Using these microphysiological systems, we demonstrate that the BBB generated fromTSC2heterozygous mutant cells shows increased permeability which can be rescued with treatment with rapamycin, an mTOR kinase inhibitor, but also by wild type astrocytes.

2024-01-16 | GSE235862 | GEO

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