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Transcriptome analysis of SREBF1 silencing and overexpression in thyroid cancer cells

ABSTRACT: Transcriptome analysis of SREBF1 silencing and overexpression in thyroid cancer cells

PROVIDER: PRJNA721465 | ENA |

REPOSITORIES: ENA

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Transcriptome analysis of SREBF1 silencing and overexpression in thyroid cancer cells

Project description:Malignant thyroid tumors have altered lipid metabolism. Sterol regulatory element-binding transcription factor 1 (SREBF1), also known as sterol regulatory element-binding protein 1 (SREBP-1), regulates cellular lipid homeostasis. We found that SREBF1 expression is a prognostic factor in patients with thyroid cancer and planned to elucidate its oncogenic mechanisms.

2021-04-13 | GSE171966 | GEO

Transcriptome analysis of CHI3L1 silencing in thyroid cancer cells

Project description:Transcriptome analysis of CHI3L1 silencing in thyroid cancer cells

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Transcriptome analysis of CHI3L1 silencing in thyroid cancer cells

Project description:CHI3L1 (Chitinase-3-like protein 1), also known as YKL-40, has been associated with inflammation and cancer. Transcription profiling analysis was performed in FTC-133 thyroid cancer cells transfected with control siRNA or siRNAs against CHI3L1.

2020-02-12 | GSE145077 | GEO

RNA sequencing analysis of SLC6A15 overexpression in papillary thyroid cancer cells

Project description:Solute Carrier Family 6 Member 15 (SLC6A15) has been found with dysregulated expression in several kinds of cancers. However, its expression pattern and biological functions in papillary thyroid cancer (PTC) remain unknown. To explore the role of SLC6A15 in PTC, transcription profiling analysis was performed in KTC1 cells with or without SLC6A15 overexpression.

2020-09-21 | GSE157646 | GEO

CREB regulated transcriptional coactivator 2 proteome landscape is plausibly modulated by SREBF1

Project description:CRTC2 is a critical transcription cofactor that induces the glucose homeostatic genes by activating CREB. However, energy homeostasis is maintained by multiple pathways, therefore, it is possible that CRTC2 may interact with other transcription factors, especially under metabolic stress. Thereby, CRTC2 liver-specific knockout mice were created and the global proteome, phosphoproteome and acetylome from liver tissue under the high fat diet conditions were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and bioinformatics analysis. As expected, differentially expressed proteins (DEPs) are enriched in metabolic pathways that were subsequently corroborated by animal experiments. The consensus DEPs from these datasets were used as seed proteins to generate a protein-protein interaction (PPI) network using STRING and GeneMANIA identified fatty acid synthase (FASN) as the mutually relevant protein. Additional local-PPI (LPPI) analysis of CRTC2 and FASN with DEPs, SREBF1 was found to be a common mediator. CRTC2/CREB and SREBF1 are transcription factors, DNA-binding motif analysis showed multiple CRTC2/CREB regulated genes also possess SREBF1 binding motifs that unveil the possible induction by CRTC2/SREBF1 complex, which is reinforced by structural analysis. Thus, CRTC2/SREBF1 complex plausibly modulate the transcription of multiple proteins that fine-tune the cellular metabolism under metabolic stress.

2023-10-24 | PXD039817 | Pride

Effect of CRABP2 depletion and overexpression on thyroid cancer cell lines

Project description:Effect of CRABP2 depletion and overexpression on thyroid cancer cell lines

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RNA sequencing analysis of SLC6A15 overexpression in papillary thyroid cancer cells

Project description:RNA sequencing analysis of SLC6A15 overexpression in papillary thyroid cancer cells

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Effects of CRABP1 overexpression on thyroid cancer cell lines

Project description:Cellular retinoic acid-binding protein 1 (CRABP1) is a cytosolic lipid-binding protein which is thought to play an important role in regulating retinoic acid signaling. Loss of CRABP1 expression has been observed in thyroid cancer. We transfected a CRABP1-overexpressing vector to thyroid cancer cells to elucidate its oncogenic effects.

2023-04-01 | GSE226035 | GEO

SREBF1 coordinates with master transcription factors in regulating lipid metabolism and cancer-promoting pathways in squamous cell carcinoma [4C]

Project description:To determine whether a TP63/KLF5-regulated super-enhancer region can impact SREBF1 transcription, circularized chromosome conformation capture (4C) assays were performed. 4C assays identified complex, extensive interactions between the SREBF1 promoter and the super-enhancer region Moreover, these DNA-DNA contacts were strictly confined within the super-enhancer region, highlighting the specificity of chromatin interactions

2021-06-26 | GSE178923 | GEO

Murine: papillary thyroid cancer vs. poorly differentiated thyroid cancer

Project description:Mice with thyroid-specific expression of oncogenic BRAF (Tg-Braf) develop papillary thyroid cancers (PTC) that are locally invasive and have well-defined foci of poorly differentiated thyroid carcinoma (PDTC). To investigate the PTC-PDTC progression, we performed a microarray analysis using RNA from paired samples of PDTC and PTC collected from the same animals by laser capture microdissection. Analysis of 8 paired samples revealed a profound deregulation of genes involved in cell adhesion and intracellular junctions, with changes consistent with an epithelial-mesenchymal transition (EMT). This was confirmed by IHC, as vimentin expression was increased and E-cadherin lost in PDTC compared to adjacent PTC. Moreover, PDTC stained positively for phospho-Smad2, suggesting a role for transforming growth factor-beta (TGFbeta) in mediating this process. Accordingly, TGFbeta induced EMT in primary cultures of thyroid cells from Tg-Braf mice, whereas wild-type thyroid cells retained their epithelial features. TGFbeta-induced Smad2 phosphorylation, transcriptional activity and induction of EMT required MAPK pathway activation in Tg-Braf thyrocytes. Hence, tumor initiation by oncogenic BRAF renders thyroid cells susceptible to TGFbeta-induced EMT, through a MAPK-dependent process. Comparing the transcription profiles of 8 pairs of murine poorly differentiated thyroid cancer and papillary thyroid cancer collected from the same animals by laser capture microdissection. Co-hybridizations were done in triplicate with a single dye flip.

2011-03-22 | E-GEOD-27668 | biostudies-arrayexpress

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