Similar Datasets
Project description:Anaerobic digestion and chain elongation in food waste fermentation
| PRJEB39281 | ENA
Project description:Although the major food-borne pathogen Campylobacter jejuni has been isolated from diverse animal, human and environmental sources, our knowledge of genomic diversity in C. jejuni is based exclusively on human or human food-chain-associated isolates. Studies employing multilocus sequence typing have indicated that some clonal complexes are more commonly associated with particular sources. Using comparative genomic hybridization on a collection of 80 isolates representing diverse sources and clonal complexes, we identified a separate clade comprising a group of water/wildlife isolates of C. jejuni with multilocus sequence types uncharacteristic of human food-chain-associated isolates. By genome sequencing one representative of this diverse group (C. jejuni 1336), and a representative of the bank-vole niche specialist ST-3704 (C. jejuni 414), we identified deletions of genomic regions normally carried by human food-chain-associated C. jejuni. Several of the deleted regions included genes implicated in chicken colonization or in virulence. Novel genomic insertions contributing to the accessory genomes of strains 1336 and 414 were identified. Comparative analysis using PCR assays indicated that novel regions were common but not ubiquitous among the water/wildlife group of isolates, indicating further genomic diversity among this group, whereas all ST-3704 isolates carried the same novel accessory regions. While strain 1336 was able to colonize chicks, strain 414 was not, suggesting that regions specifically absent from the genome of strain 414 may play an important role in this common route of Campylobacter infection of humans. We suggest that the genomic divergence observed constitutes evidence of adaptation leading to niche specialization. Data is also available from <ahref=http://bugs.sgul.ac.uk/E-BUGS-95 target=_blank>BuG@Sbase</a>
2011-04-15 | E-BUGS-95 | biostudies-arrayexpress
Project description:The MFP2-/- (multifunctional protein 2) is a multifunctional enzyme with discovery in connection with different metabolic pathways. It is involved in beta-oxidation of branched fatty acids and long chain fatty acids in peroxisomes and in sythesis of bile fatty acids. MFP2 knock out mouse was made with a phenotype only visible in homozygotes (severe growth retardation, 40% mortality during the first week after birht, reduction of fertility in males, less activity). The studies have been performed to identify changes in gene expression levels in kidney due to clinical chemical changes in the phenotype of this mutant mouse line during a screening in the German Mouse Clinic. Keywords: dual colour hybridisation on cDNA microarrays, MFP2, Hsd17b4, kidney
2008-06-01 | GSE6295 | GEO