Project description:Environmental extracts and fractions obtained with passive sampling (HP-20, SMIRC) from Mission Bay, Point Loma, Scripps Pier, Salton Sea (San Diego and Imperial Counties)
2023-10-18 | MSV000093148 | MassIVE
Project description:16S rRNA amplicon sequencing in the Hudson Bay estuaries
Project description:This dataset includes the Fastq files from sequencing data generate from diagnostic and remission bone marrow mononuclear cell samples using the Mission Bio Tapestri Plateform with both DNA amplicons and protein from antibody-derived tags sequencing libraries.
Project description:Environmental extracts and fractions obtained with passive sampling (HP-20, SMIRC) from Mission Bay, Point Loma, Scripps Pier, Salton Sea (San Diego and Imperial Counties)
Project description:Amplicon-based targeted re-sequencing analysis was performed in the patient-derived gliobastoma cell culture samples. For this purpose, genomic DNA (gDNA) was isolated and DNA libraries were prepared using the TruSeq Custom Amplicon Low Input (Illumina, Inc.) technology. By this, a pool of 375 amplicons was generated for each single sample in order to enrich for the target genes ATRX1, EGFR, IDH1, NF1, PDGFRA, PIK3CG, PIK3R1, PTEN, RB1 and TP53. Sequencing was performed on the Illumina MiSeq® next generation sequencing system (Illumina Inc.) and its 2 x 250 bp paired-end v2 read chemistry. The resulting reads were quality controlled and mapped against the human reference genome (hg19). For all samples, sequence variations of the amplified regions of interest in comparison to the human reference sequence were identified and filtered based on reliability.
Project description:RNA-sequencing (Ribo-ZERO, Illumina HiSeq-2500, single-end 50 bp reads) was on human glioblastoma stem cells (B67) performed 5 days after lentivirus transduction with MISSION TRC lentiviruses using control scrambled (sh002) or two distinct TRIM26 RNAi (TRCN0000004050 (sh4050) and TRCN0000004051 (sh4051)).
