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Nonlinear control of transcription through promoter-enhancer interactions [splinkerette PCR]

ABSTRACT: Nonlinear control of transcription through promoter-enhancer interactions [splinkerette PCR]

PROVIDER: PRJNA722528 | ENA |

REPOSITORIES: ENA

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Project description:Although the locations of promoters and enhancers have been identified in several cell types, we have yet limited information on their connectivity. We developed HiCap, which combines Hi-C with promoter sequence capture, to enable genome-wide identification of regulatory interactions with single-enhancer resolution. HiCap analyses of mouse embryonic stem cells (mESC) identified promoter-enhancer interactions predictive of gene expression change upon perturbation, opening up for genomic analyses of long-range gene regulation. HiCap was designed by combining Hi-C with with sequence capture (for all promoters) and carried out in mouse embryonic stem cells (mESC)

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